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| 凝胶萃取浓缩分离大分子物质 | |
| 引用本文： | 陈先法,施亚钧. 凝胶萃取浓缩分离大分子物质[J]. 医学教育探索, 1991, 0(4) |
| 作者姓名： | 陈先法 施亚钧 |
| 作者单位： | 上海水产大学食品科学技术系(陈先法)，华东化工学院化学工程研究所(施亚钧) |
| 摘 要： | 用四种不同交联度的交联聚丙烯酰胺凝胶,研究了它们在去离子水及牛血清白蛋白、牛血红蛋白、精氨酸酶、聚乙二醇等大分子溶液中的溶胀速率和平衡溶胀比的变化规律。发现其平衡溶胀比越大,溶胀速率也越快;凝胶在大分子溶液中的平衡溶胀比总是小于它在水中的值,且随大分子溶液浓度增大,平衡溶胀比的减小程度增大;温度升高,溶胀速率和平衡溶胀比均增大。凝胶交联度越大,对大分子物质的分离效率越高,浓缩精氨酸酶时的最大值为53.4%。凝胶的再生可用加少量盐或丙酮或调节pH值等方法。 |
| 关 键 词： | 凝胶 浓缩 分离 大分子 凝胶萃取 交联聚丙烯酰胺凝胶 |
Concentration and Separation of Macromolecular Solution by Gel Extraction |
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| Chen Xianfa Shi Yajun. Concentration and Separation of Macromolecular Solution by Gel Extraction[J]. Researches in Medical Education, 1991, 0(4) | |
| Authors: | Chen Xianfa Shi Yajun |
| Affiliation: | Chemical Engineering Research Center |
| Abstract: | The changing rules of swelling speed and equilibrium swelling degree of four polyacrylamide gels with different cross-linkages in deionized water as well as macromolecular solutions of polyethylene glycol, brovine serum albumin, brovine blood hemoglobin and brovine liver arginase were studied. The effect of gel cross-linkage on the separation efficiency of arginase was explored preliminarily. The regeneration performance of gels was discussed too. These experimental results offer a base for further research on gel extraction separation technology. |
| Keywords: | gel concentration separation macromolecule gel extraction cross-linked polyacrylamide gel |
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