A monoclonal antibody recognizing a determinant common to HLA—A3 and All |
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| Authors: | R. Billing B. Clark K. Lucero B. J. Shi P. Terasaki |
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| Affiliation: | Department of Surgery, UCLA School of Medicine, University of California, Los Angeles, California, U.S.A. |
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| Abstract: | ![]()
Spleen cells from a mouse immunized with an AML cell expressing HLA-A3 produced a hybridoma secreting an anti-HLA-A3, A11 monoclonal antibody, 26D3. By complement-dependent cytotoxicity at dilutions to 1:10(4) the ascites antibody lysed 13/13 A3, 15/15 A11, and 8 other lymphocytes from a panel of 98 donors. The 26 D3 immunoprecipitated a molecule consisting of subunits of 44,000 and 12,000 daltons. Monoclonality of the antibody was demonstrated by isoelectric focusing and protein A affinity chromatography. |
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