鳞癌相关抗原在诱发性肺鳞癌癌变过程中动态监测的研究

 Squamous cell c目的　探讨肺癌形成过程中血清鳞癌相关抗原 (SCCAg)、癌胚抗原 (CEA)及糖链抗原15 3(CA15 3)的动态变化 ,为寻找肺癌早期诊断指标提供依据。方法　经肺叶支气管内灌注甲基胆蒽 (MCA)碘油溶液对 91只Wistar大鼠诱癌 ,分别于灌注后第 2 0天、4 0天、50天、60天、70天、80天分批处死动物并取血清。从经病理确诊为不典型增生、原位癌及早期浸润癌各阶段的动物模型组中各选取 3～ 7例 ,应用微粒子酶免疫试验技术 (MEIA)测定血清中SCCAg、CEA、CA15 3的水平。结果　在不典型增生、原位癌阶段 ,三种标志物均无改变 ;但在早期浸润癌阶段 ,SCCAg明显增高 ,其差异有显著意义 (P <0 0 1) ,而CEA、CA15 3无明显改变。结论　血清SCCAg在肺鳞癌早期即升高 ,表明SCCAg是肺鳞癌早期诊断的一个很有意义的指标。

Study on Change of Squamous Cell Carcinoma Antigen of Model Rat of Lung Squamous Cell Carcinoma

Objective To evaluate significance of squamous cell carcinoma associated antigen (SCC Ag)in early diagnosis for the squamous cell carcinoma of the lung.Methods The squamous cell carcinoma of lung was induced with methylcholanthrene(MCA)in iodized oil in wistar rats.Ninty-one rats were used for this experiment,and the rats were sacrificed on the 20th,40th,50th,60th,70th and 80th day respectively.The stage of the cancer development including atypical hyperplasia,carcinoma in situ,early invasive carcinoma and infiltrating cancer were diagnosed histopathologically. SCC Ag,CEA and CA15-3 in the serum of different conceration stage rats were measured by MEIA technique.Results The level of SCC Ag increased distinctly ( P <0.01) at the early invasive carcinoma,but the other two markers had no change.Conclusion It appears that SCC Ag level in serum may be of considerable importance in early diagnosis for the squamous cell carcinoma of the lung.