他克莫司及阿霉素对肝癌细胞抑制作用的实验研究

摘要：目的:探讨他克莫司(FK506)及阿霉素（ADM）对肝癌细胞的影响。方法:培养HepG-2肝癌细胞株，将其分成对照组、FK506组、ADM及FK506加ADM组，分别处理24~48h。采用MTT法检测细胞增殖，流式细胞术测定细胞周期及细胞凋亡。结果:肝癌细胞增殖能力在FK506作用48h后明显受到抑制；FK506对肝癌细胞有促进凋亡作用，在5~100μｇ/L浓度范围内，凋亡率随浓度增加而增加，超过100μｇ/L时,凋亡率减低。FK506及ADM均使G2期显著延长;FK506联合ADM对G2/M期的阻滞有协同作用。FK506与ADM有协同促凋亡作用。结论:FK506对肝癌细胞的增殖有抑制作用,能使G2期阻滞,促凋亡。FK506与ADM体外联合应用对肝癌细胞有协同抑制作用。

Inhibitory effect of combined tacrolimus and adriamycin on hepatocellular carcinoma:an experimental study

Abstract:Objective:To investigate the inhibitiory effect of tacrolimus(FK506) and adriamycin(ADM) on hepatocellular carcinoma. Methods:HepG-2 cells were cultured, and divided into 4 groups, namely control、FK506、ADM、FK506+ADM groups, the cells were trated by the drugs for 24 to 48 hours respectively. The inhibitory rate of the cells was measured by MTT assay, and cell cycle and cell apoptotic rate were detected by flow cytometry(FCM). Results:The ability of tumor cell growth were inhibited by FK506 after 48h; the apoptosis ratio was increased when FK506 was below 100μｇ/L, and when it exceeded that value, the apoptosis ratio was decreased. FK506 and ADM significantly prolonged cell G2 phase; combined tacrolimus and adriamycin had synergistic role effect on arresting the cell in G2 phase, FK506 combined with adriamycin demonstrated synergistic effect on apoptosis. Conclusions:FK506 could inhibit the growth of hepatocellular carcinoma, arrest the cell in G2 phase, and increase apoptosis. FK506 combined with adriamycin demonstrated synergistic inhibitory effect on hepatocellular carcinoma.