| 不同产地刺玫果中总黄酮含量的测定 |
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| 引用本文： | 王领弟,李艳荣,潘海峰,张晓峰. 不同产地刺玫果中总黄酮含量的测定[J]. 承德医学院学报, 2012, 29(1): 5-7 |
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| 作者姓名： | 王领弟 李艳荣 潘海峰 张晓峰 |
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| 作者单位： | 王领弟 (河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000) ； 李艳荣 (河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000) ； 潘海峰 (河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000) ； 张晓峰 (河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000) ； |
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| 摘 要： | 目的:建立刺玫果中总黄酮含量的测定方法。方法:以芦丁为对照品,采用紫外-可见分光光度法在波长510nm处测定刺玫果中总黄酮的含量。结果:芦丁在8.0-48mg/L范围线性关系良好,回归方程为Y=12.602X-0.0216(r=0.9996)。不同产地刺玫果样品中总黄酮的含量为1.593%-3.742%,差异较大。结论:本研究建立的方法准确、可靠、灵敏,可作为刺玫果中总黄酮含量的测定方法。
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| 关 键 词： | 刺玫果 总黄酮 含量测定 紫外-可见分光光度法 |
DETERMINATION OF TOTAL FLAVONOIDS CONTENTS IN ROSA DAVURICA PALL FROM DIFFERENT PRODUCING AREAS |
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| Affiliation: | WANG Ling-di,LI Yan-rong,PAN Hai-feng,et al(Hebei Key Laboratory of Study and Exploitation of Chinese Medicine,Institute of Chinese Materia Medica,Chengde Medical College,Hebei Chengde 067000,China) |
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| Abstract: | Objective:To establish the determination methods of total flavonoids contents in Rosa Davurica Pall.Methods:Ultraviolet and visible spectrophotometry was used to determine the total flavonoid contents in Rosa Davurica Pall at wavelength 510nm by taking rutin as standard substance.Results:Rutin had good linear relationship at the range 8.0-48 mg/L and the regression equation was Y=12.602X-0.0216(r=0.9996).The total flavonoids contents in Rosa Davurica Pall from different producing area was 1.593%-3.742%,had fairly large difference.Conclusions:This method is accurate,reliable and sensitive;and can be acted as methods for determining total flavonoids contents in Rosa Davurica Pall. |
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| Keywords: | Rosa Davurica Pall Total flavonoids Content determination Ultraviolet and visible spectrophotometry |
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