Effectiveness of animal rotation in achieving uniform dust exposures and lung dust deposition in horizontal flow chambers

An important source of variability of experimental results in inhalation toxicology originates from the nonuniformity of chamber concentrations. Two horizontal flow inhalation chambers were used in evaluating the effect of cage rotation within each chamber on the amount of alpha-quartz deposited in the lungs of male Fischer 344 rats. The alpha-quartz lung burden was determined by quantitative X-ray diffraction. A carefully designed animal rotation schedule significantly reduced the variability in the amount of alpha-quartz deposited in the animals, thereby minimizing effects of chamber nonuniformity due either to design or dust generator characteristics.