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| 不同浓度纳米银对血管内皮细胞增殖作用的影响 | |
| 引用本文： | Cui J,Zhang YD. 不同浓度纳米银对血管内皮细胞增殖作用的影响[J]. 细胞与分子免疫学杂志, 2011, 27(6): 697-699 |
| 作者姓名： | Cui J Zhang YD |
| 作者单位： | 中南大学卫生部肝胆肠外科研究中心,湖南,长沙,410000 |
| 摘 要： | 目的:观察不同浓度纳米银对体外培养血管内皮细胞增殖的影响.方法:取3只5 d龄SD大鼠,分离心脏,在体外酶消化法分离血管内皮细胞并进行培养,第3代细胞用于实验.除空白对照组外,血管内皮细胞分别于含0.5,0.25,0.125,0.0625,0.03125 g/L纳米银的培养基中进行培养.24 h后倒置显微镜下观察细胞形态并进行细胞计数,采用甲基噻唑基四唑比色法和流式细胞术检测血管内皮细胞的增殖情况,并采用LDH法检测急性细胞毒性.结果:与空白对照组比较,不同浓度的纳米银组血管内皮细胞细胞数减少,纳米银浓度为0.25 g/L时,对血管内皮细胞的抑制作用最明显,其他浓度彼此间无统计学差异.不同浓度纳米银对血管内皮的增殖均有一定抑制作用,但并没有急性细胞毒性.结论:纳米银对体外培养的血管内皮细胞有明显的抑制增殖作用,其作用与纳米银的剂量有关. |
| 关 键 词： | 纳米银 血管内皮细胞 细胞增殖 |
Effects of different doses of nano silver on vascular endothelial cell proliferation in vitro |
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| Cui Jian,Zhang Yang-de. Effects of different doses of nano silver on vascular endothelial cell proliferation in vitro[J]. Chinese journal of cellular and molecular immunology, 2011, 27(6): 697-699 | |
| Authors: | Cui Jian Zhang Yang-de |
| Affiliation: | CUI Jian,ZHANG Yang-de Hepatobiliary & Enteric Surgery Research Center,Central South University,Changsha 410000,China |
| Abstract: | AIM: To explore the effects of different doses of nano silver on proliferation of cultured vascular endothelial cells in vitro.METHODS: The hearts of thre e new-born SD rats 5 days old were mechanically minced and enzymatically digested with collagenase and trypsin,then vascular endothelial cells were counted,washed and resuspended in Dulbecco's minimumes sential medium(DMEM) added with 20% heat in activated fetal calf serum,then inoculate d in 2% gelatin coated tissue culture flasks.Vascular endothelial ce... |
| Keywords: | nano silver vascular endothelial cell cell proliferation |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |