镁合金材料的溶血率及影响因素

背景:镁合金作为潜在的新型医用可降解生物金属材料受到越来越多的关注,体外纯镁合金浸提液具有溶血作用.目的:探讨镁合金浸提液与其溶血率的关系.方法:将镁合金浸提液稀释,按GBT 16175-2008<医用有机硅材料生物学评价实验方法>第13 部分<溶血试验>的改良方法进行溶血实验.测量A值,计算溶血率,各稀释度溶液钠、镁离子浓度及pH值.结果与结论:100%,50%,25%,10%,5%,2.5%和1%稀释度镁浸提液的溶血率分别为65.3%,66.7%,33.8%,11.8%,6.2%,0.3%和-0.3%.随着稀释度的加大,镁离子浓度呈下降趋势,而钠离子则无明显波动.100%,50%,25%,10%,5%和2.5%稀释度之间的pH值差异无显著性意义(P>0.05),均高于1%稀释度(P<0.05).提示镁合金浸提液的高pH值和离子浓度是影响其体外试验溶血的主要因素.

Haemolyticus of manalium alloy and influencing factors

BACKGROUND:Magnesium alloy is a new potential medical biodegradable metal material has been obtained more and more attention;in vitro pure magnesium alloy leaching liquor has hemolysis.OBJECTIVE:To evaluate the relationship between pure magnesium alloy leaching liquor and its haemolyticus.METHODS:Magnalium alloy leaching liquor was diluted with normal sodium.Haemolysis test was preformed with the improved method which mentioned in the "Haemolysis Test",the 13 th part of "Experimental Methods for the Biological Evaluation of Medical Silicone Materials".The absorbance value was measured,the haemolyticus was calculated,and the concentration of sodium,magnesium ion in dilution solution and the pH value was detected.RESULTS AND CONCLUSION:Haemolyticus of the magnesium alloy leaching liquor with 100%,50%,25%,10%,5%,2.5% and 1% dilution solution was 65.3%,66.7%,33.8%,11.8%,6.2%,0.3% and-0.3%,respectively.With the increased concentration of dilution solution,the concentration of magnesium ion was decreased,but the concentration of sodium ion was not changed obviously.There was no difference in the pH value of 100%,50%,25%,10%,5% and 2.5% dilution solution(P > 0.05),and all the pH value in these group was higher than that in 1% dilution solution group(P < 0.05).High pH value and ionic concentration in magnalium leaching liquor were the main factors of haemolysis test in vitro.