血管内皮生长因子与碱性成纤维细胞生长因子联合应用对自体游离脂肪颗粒移植成活的影响

背景:微血管的生成是脂肪细胞成活的关键。研究显示血管内皮生长因子与碱性成纤维细胞生长因子分别对血管生长有促进作用。目的:观察联合应用血管内皮生长因子与碱性成纤维细胞生长因子对脂肪颗粒移植成活的影响。方法:50只SD大鼠随机分为5组,将自体大网膜脂肪颗粒移植于背部,然后分别加入生理盐水,50μg/L血管内皮生长因子,50μg/L碱性成纤维细胞生长因子,50μg/L血管内皮生长因子+50μg/L碱性成纤维细胞生长因子,100μg/L血管内皮生长因子+100μg/L碱性成纤维细胞生长因子。于移植15,30d处死大鼠取出移植物。结果与结论:100μg/L血管内皮生长因子+100μg/L碱性成纤维细胞生长因子实验组移植物的残余质量和微血管密度明显高于其他组,差异有显著性意义(P<0.05),其他各组间差异无显著性意义(P>0.05)。结果可见血管内皮生长因子与碱性成纤维细胞生长因子联合应用可促进移植的脂肪颗粒成活。

Effect of vascular endothelial growth factor and basic fibroblast growth factor on the survival of autologous free fat particles after transplantation

BACKGROUND:The micro-angiogenesis is the key to the survival of the fat cells.Research shows vascular endothelial growth factor and basic fibroblast growth factor can promote blood vessel growth.OBJECTIVE:To observe the effect of vascular endothelial growth factor and basic fibroblast growth factor on the survival of fat particles after transplantation.METHODS:Fifty SD rats were divided into five groups,and the autologous greater omentum was transplanted to the rat back,and then added normal saline,50 μg/L vascular endothelial growth factor,50 μg/L basic fibroblast growth factor,50 μg/L vascular endothelial growth factor+50 μg/L basic fibroblast growth factor and 100 μg/L vascular endothelial growth factor+100 μg/L basic fibroblast growth factor,respectively.At 15 and 30 days after transplantation,rats were killed to remove the graft.RESULTS AND CONCLUSION:The graft residual weight and vascular density in 100 μg/L vascular endothelial growth factor+100 μg/L basic fibroblast growth factor experimental group was significantly higher than those in the other three groups and the difference was significant (P < 0.05),there was no significant difference among the other four groups (P > 0.05).The combination of vascular endothelial growth factor and basic fibroblast growth factor can significantly promote the fat particles to survive.