痛风颗粒干预腺嘌呤、乙胺丁醇所致高尿酸性肾病模型大鼠肾脏形态学改变

背景:课题组通过长期、大量的临床实践,将痛风颗粒用于治疗尿酸性肾病及痛风性关节炎,取得了良好的疗效。目的:观察痛风颗粒对高尿酸性肾病模型大鼠肾脏病理形态学改变的影响。方法:66只SD大鼠随机分为6组,正常对照组、模型组、痛风颗粒高、中、低剂量组、苯溴马隆组。除正常对照组外,其余5组SD大鼠分别制作高尿酸血症性肾病动物模型,同时各组分别予以痛风颗粒或苯溴马隆灌胃治疗,正常对照组及模型组给予相同容量的生理盐水,连续灌服21d。结果与结论:与模型组相比,治疗组和正常对照组肾脏质量显著降低,体质量增加明显(P<0.05)。正常对照组肾组织结构正常;模型组可见局灶性间质纤维化,伴大量淋巴和单核细胞的浸润;痛风颗粒高剂量组极少见间质纤维化,伴有少量淋巴和单核细胞的浸润;中剂量组可见少数间质纤维化,伴有少量淋巴和单核细胞的浸润;低剂量组可见数量较多的间质纤维化,伴有大量淋巴和单核细胞的浸润;苯溴马隆组可见少量间质纤维化,伴有大量淋巴和单核细胞浸润。提示痛风颗粒能有效抑制高尿酸模型动物肾脏的炎症,而且能够减轻肾脏肾间质纤维化和肾小球硬化。

Effects of tongfeng instant powder on renal pathological changes in adenine and ethambutol-induced hyperuricemia nephropathy rats

BACKGROUND:Over the years,we have obtained a good effect of tongfeng instant powder on uric acid nephropathy and gouty arthritis through long-term and large-amount clinical practice.OBJECTIVE:To observe the pathological changes of the kidneys in rats with hyperuricemia nephropathy after the treatment with tongfeng instant powder.METHODS:Sixty-six Sprague-Dawley rats were randomized into six groups:normal control group,model group,benzbromarone group,high-,middle-,and low-dose tongfeng instant powder groups.Animal models of hyperuricemia nephropathy were established in the latter five groups.All groups were treated with tongfeng instant powder or benzbromarone or the same volume of saline solution per day until day 21 after modeling.RESULTS AND CONCLUSION:Compared with the model group,the renal mass was reduced significantly,while the body mass was increased significantly in the treatment groups and normal control group(P < 0.05).The renal structure in the normal control group maintained normal.There was focal interstitial fibrosis in the model group,with a large number of infiltrated lymphoid tissue and monocytes.In the high-dose group,rare interstitial fibrosis was seen,accompanied by a small amount of infiltrated lymphoid tissues and monocytes.In the middle-dose group,a little interstitial fibrosis was seen,accompanied by a small amount of infiltrated lymphoid tissue and monocytes.In the low-dose group,a great amount of interstitial fibrosis was found with a large amount of infiltrated lymphoid tissue and monocytes.In the benzbromarone group,a small amount of interstitial fibrosis was observed,accompanied by a large number of infiltrated lymphoid tissue and monocytes.These findings indicate that tongfeng instant powder can effectively suppress the renal inflammation of hyperuricemia nephropathy animal models and reduce renal interstitial fibrosis and glomerular sclerosis.