| HPLC法测定七厘散中儿茶素和表儿茶素的含量 |
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| 引用本文： | 谭生建,贺业谦,李勉珊,史宁,薛克昌.HPLC法测定七厘散中儿茶素和表儿茶素的含量[J].解放军药学学报,2012,28(1):72-73,76. |
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| 作者姓名： | 谭生建 贺业谦 李勉珊 史宁 薛克昌 |
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| 作者单位： | 1. 解放军306医院药学部,北京,100101
2. 北京黄寺美容外科医院,北京,100011 |
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| 摘 要： | 目的建立七厘散中儿茶素和表儿茶素的含量测定方法。方法 HPLC法,采用Prevail Select C18(150 mm×4.6mm,5μm)色谱柱,柱温:35℃;以乙腈-水-磷酸(100∶900∶1)为流动相;流速:1 ml/min;检测波长:278 nm。结果儿茶素、表儿茶素线性浓度范围分别为30.08～150.4μg/ml,r=0.9999;12.24～61.20μg/ml,r=0.9999;回收率分别为99.1%和97.4%,RSD分别为1.1%和1.2%。结论本方法操作简便,测定结果准确可靠,可用于七厘散中儿茶素和表儿茶素的含量测定。
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| 关 键 词： | HPLC法 七厘散 儿茶素 表儿茶素 |
Determination of Catechin and Epicatechin in Qili San by HPLC |
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| TAN Sheng-jian
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HE Ye-qian
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LI Mian-shan
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SHI Ning
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XUE Ke-chang.Determination of Catechin and Epicatechin in Qili San by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2012,28(1):72-73,76. |
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| Authors: | TAN Sheng-jian HE Ye-qian LI Mian-shan SHI Ning XUE Ke-chang |
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| Institution: | ① ①Department of Pharmacy,Hospital 306 of PLA,Beijing 100101,China; ②Beijing Huangsi Cosmetic Surgery Hospital,Beijing 100011,China |
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| Abstract: | Objective To establish a method for determining catechin and epicatechin in Qili San.Methods HPLC was used for the determination.The chromatographic column was Prevail Select C18（150 mm ×4.6 mm,5 μm）and the column temperature was 35℃.The mobile phase consisted of acetonitrile-water-phosphoric acid（100∶ 900∶ 1）.The flow rate was 1 ml/min and monitored at 278 nm.Results The linear range of catechin was 30.08-150.4μg/ml,r=0.9999.The average recovery of catechin was 99.1% and RSD was 1.1%.The linear range of epicatechin was 12.24-61.20μg/ml,r=0.9999.The average recovery of epicatechin was 97.4% and RSD was 1.2%.Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of catechin and epicatechin in Qili San. |
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| Keywords: | HPLC Qili San catechin epicatechin |
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