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| 基于ITS2序列的积雪草及其混伪品的分子鉴定 | |
| 引用本文： | 陈晓辰,陈倩,韩建萍. 基于ITS2序列的积雪草及其混伪品的分子鉴定[J]. 中药研究与信息, 2012, 0(3): 12-14 |
| 作者姓名： | 陈晓辰 陈倩 韩建萍 |
| 作者单位： | [1]濒危药材等高育国家工程实验室,中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]湖北中医药大学,湖北武汉430061 |
| 摘 要： | 目的：快速准确的鉴别中药积雪草及其混伪品。方法：采用ITS2序列片段，对其进行PCR扩增、测序，运用Codon CodeAligner、MEGA4．1等软件进行序列拼接和分析，构建积雪草及其混伪品的NJ树。应用Koetschan等建立的ITS2数据库及其网站预测ITS2二级结构。结果：积雪草及其混伪品ITS2序列之间存在明显差异，不同积雪草样品在NJ树上独立聚为一支。结论：运用ITS2序列可以准确鉴定积雪草及其伪品。 |
| 关 键 词： | 积雪草 ITS2 混伪品 分子鉴定 |
Molecular Identification of Centella asiatica and Its Adulterants Using ITS2 DNA Barcode |
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| CHEN Xiao-ehen,CHEN Qian,HAN Jian-ping. Molecular Identification of Centella asiatica and Its Adulterants Using ITS2 DNA Barcode[J]. Research & Information on Traditional Chinese Medicine, 2012, 0(3): 12-14 | |
| Authors: | CHEN Xiao-ehen CHEN Qian HAN Jian-ping |
| Affiliation: | 1 ( 1. National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Belting 100193, China; 2. Hubei Univercity of Chinese Medicine, Wuhan 430061, China ) |
| Abstract: | Objective：To identify Centella asiatica and its adulterants in a fast and specific way. Methods： ITS2 regions as DNA bareode were amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. Sequence analyses were carried out by MEGA4. 1 software. The phylogenetie tree was constructed using the neighbor-joining and Kimura 2-parameter methods. Results： A significant difference was observed in the ITS2 sequences between Centella asiatica and its adulterants. Inter-specific genetic distance is higher than intra-speeifie one, sequences and secondary structure of ITS2 can distinguish Centella asiatica from its adulterants. Conclusion： ITS2 can serve as a valuable marker for identifying Centella asiatica and its adulterants. |
| Keywords: | Centella asiatica ITS2 adulterants Molecular identification |
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