枳椇子提取物对大鼠抗疲劳作用的比较

目的研究枳椇子乙酸乙酯萃取物对跑台运动大鼠的抗疲劳作用,并比较枳椇子乙酸乙酯萃取物和枳椇子水提醇沉提取物的抗疲劳作用。方法除正常组外,所有实验动物每天进行30 min速度为15 m/min的跑台训练,连续7 d;同时分别灌胃给予枳椇子乙酸乙酯萃取物水混悬液4、8、12 mg/(kg.d);枳椇子水提醇沉提取物水混悬液300 mg/(kg.d);诺迪康胶囊280 mg/(kg.d)(阳性药物组);模型组与对照组分别灌胃给予等体积饮用水(10 ml/kg),连续7 d;于第8天进行1 h速度为25 m/min的跑台运动;跑台结束后,立即采集血液、肝脏、肌肉和脑组织,检测血清肌酸激酶、血清乳酸脱氢酶、血清乳酸、血清乙酰胆碱酯酶、血清尿素氮、血清总抗氧化力、血糖、血红蛋白、脑超氧化物歧化酶、脑丙二醛、肝糖原、肌糖原等指标。结果模型组与对照组比较,各检测指标有显著性差异;枳椇子乙酸乙酯萃取物给药组大鼠的各项指标得到明显改善,中、高剂量组各项指标改善状况优于枳椇子水提醇沉组。结论枳椇子乙酸乙酯萃取物对跑台运动大鼠具有显著的抗疲劳作用,并优于枳椇子水提醇沉物。

The Study of Hovenia Dulis Thumb Anti-fatigue Effect on Rats Taking Movement

Objective To study the effect of Hovenia dulis thumb on rats′ ability to move.Methods Except the normal group,all animals were trained 30 min per day for 7 d at the speed of 15 m/min and then run at the speed of 25 m/min at the 8th day for 1 h.During these seven days,animals were givenig water suspension of Hovenia dulis thumb ethyl acetate extract respectively at 4,8,12 mg/（kg·d）.The positive control group was given water suspension of Nuodikang,while the model and control groups were given water at 10 ml/kg weight.After running at the 8th day,all animals had their blood,liver,muscle and brain tissue collected.Then according to the kit′ introductions,the content of indices,such as serum creatine kinase,serum lactate dehydrogenase,serum lactate,serum ache,serum urea nitrogen,serum total antioxidant capacity,glucose,hemoglobin,superoxide dismutase brain,brain mda,liver glycogen,and muscle glycogen were tested.Results Indices of model group and normal group were significantly different.Indices of animals given Hovenia dulis thumb extract were improved.Conclusion Hovenia dulis thumb ethyl acetate extract has great anti-fatigue effect,better than that of Hovenia dulis thumb ethanol extract.