干细胞分泌因子促进难愈性创面愈合的机制及应用前景

背景：干细胞能够分泌多种伤口愈合相关因子。目的：总结并分析干细胞分泌的创面愈合相关因子治疗难愈性创面的可行性,及其可能的调控及修复机制。方法：应用计算机检索Google、Pubmed及万方数据库相关文献,英文检索词为＂chronic wound,refractory wound,cutaneous,wound healing,wound repair,diabetic wound,stem cell-derived conditioned medium,cellular cytokine solution＂。中文检索词为＂创面修复,创伤修复＂。选择论点论据可靠且分析全面的与干细胞分泌的促进伤口愈合因子密切相关的文章,排除内容重复文献,最终纳入34篇文献进行总结综述。结果与结论：多种干细胞培养上清液中富含创面愈合相关因子,可作为细胞因子制剂治疗难愈性创面。然而由于干细胞来源不同,体外培养条件不同,其因子分泌状况也有所差异,因此应用何种来源的干细胞分泌因子,如何在体外最大限度地刺激干细胞产生创面愈合相关因子以及其未来在临床应用中的使用时机、频次和剂量仍有待进一步探索。

The future perspective and proposed molecular mechanism of stem cell-secreted factors in promoting refractory wound healing

BACKGROUND： Stem cells secrete numerous types of wound healing related factors. OBJECTIVE： To review the feasibility of stem cell-secreted factors in the treatment of refractory wound, and the possible regulation and repairing mechanism. METHODS： A computer-based online search of Google, Pubmed Database and Wanfang Database were performed for articles and reviews. The key words were ＂chronic wound, refractory wound, cutaneous, wound healing, wound repair, diabetic wound, stem cell-derived conditioned medium, cellular cytokine solution＂ in English, and ＂would repair, injury repair＂ in Chinese. A total of 57 articles were retrieved, and screened following reading titles and abstracts. Literatures about stem cell-secreted wound healing related factors were included. Repetitive and poor quality articles were excluded. Finally, 34 literatures were included. RESULTS AND CONCLUSION： Stem cell-derived conditioned medium contains numerous wound healing related factors, which can be used as an alternative in treating refractory wound. However, stem cells from different sources or in different cultivation conditions will produce different secretions, thus further exploration is needed to resolve issues such as which kind of stem cells should be used to generate conditioned medium, how to stimulate stem cells to largely produce wound healing related factors, how frequent and at what dosage and timing should stem cell-derived conditioned medium be optimally applied.