| 乙醇改变骨髓间充质干细胞神经肽的表达 |
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| 引用本文： | 王义生,朱绍阳,李月白,赵国强. 乙醇改变骨髓间充质干细胞神经肽的表达[J]. 中国临床康复, 2012, 0(6): 981-984 |
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| 作者姓名： | 王义生 朱绍阳 李月白 赵国强 |
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| 作者单位： | [1]郑州大学第一附属医院骨科,河南省高等学校临床医学重点学科开放实验室,河南省郑州市450052 [2]郑州大学基础医学院生物化学教研室,河南省郑州市450001 [3]郑州大学基础医学院微生物免疫教研室,河南省郑州市450001 |
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| 基金项目： | 国家自然科学基金资助项目（81171776） 课题名称：hCGRPα基因修饰BMSCs联合自体骨移植治疗股骨头坏死的实验研究~~ |
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| 摘 要： | 背景：研究发现骨髓间充质干细胞的分化受神经系统调控。目的：观察乙醇对人骨髓间充质干细胞降钙素基因相关肽受体、P物质受体、过氧化物酶体增殖子活化受体γmRNA及Runx2mRNA表达的影响。方法：将第2代人骨髓间充质干细胞随机分组培养：实验组加入0.09mol/L乙醇,对照组正常培养。结果与结论：培养11d后,实验组降钙素基因相关肽受体、P物质受体、Runx2mRNA表达较对照组明显降低（P〈0.01）,过氧化物酶体增殖子活化受体γmRNA水平较对照组明显增高（P〈0.01）。表明乙醇改变了骨髓间充质干细胞中神经肽类物质的表达,减弱了其向成骨细胞方向分化。
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| 关 键 词： | 乙醇 神经肽 人源性骨髓间充质干细胞 降钙素基因相关肽 P物质 |
Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells |
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| Wang Yi-sheng,Zhu Shao-yang,Li Yue-bai,Zhao Guo-qiang. Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells[J]. Chinese Journal of Clinical Rehabilitation, 2012, 0(6): 981-984 |
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| Authors: | Wang Yi-sheng Zhu Shao-yang Li Yue-bai Zhao Guo-qiang |
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| Affiliation: | , Zhang Zhan-feng 1Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Key Subjects, Zhengzhou 450052, Henan Province, China; 2Department of Biochemistry; 3Department of Microbiology ＆ Immunology, Basic Medical College of Zhengzhou University, Zhengzhou 450001, Henan Province, China |
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| Abstract: | BACKGROUND： Studies have found that the nervous system can effect the differentiation of bone marrow mesenchymal stem cells （BMSCs）. OBJECTIVE： To investigate the effect of alcohol on the expressions of calcitontin gene-related peptide receptor （CGRPR） mRNA, substance P receptor （SPR） mRNA, peroxisom proliferator-activeted receptor-γ （PPARγ） mRNA and Runx2 mRNA in human BMSCs. METHODS： The second generation of BMSCs were divided into two groups at random. The cells in experimental group were treated with 0. 09 mol/L alcohol, and the control group was not treated. RESULTS AND CONCLUSION： The expressions of FGF mRNA, CGRPR mRNA, SPR mRNA and Runx2 mRNA in the experimental group were significantly lower those that in the control group （P〈0.01）, but the expression of PPARγ mRNA was increased compared with control group （P〈0.01）. Alcohol could change the expressions of neuropeptide and depress the differentiation to osteoblasts. |
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