| 脐血来源间充质干细胞可抑制异体T细胞的反应 |
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| 引用本文： | 李志斌,曹引丽,刘嗣超,董莉洁,鲁会英.脐血来源间充质干细胞可抑制异体T细胞的反应[J].基础医学与临床,2011,31(10). |
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| 作者姓名： | 李志斌 曹引丽 刘嗣超 董莉洁 鲁会英 |
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| 作者单位： | 陕西省妇幼保健院,陕西西安,710003 |
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| 摘 要： | 目的研究脐血间充质干细胞(HUCB-MSCs)对异体T细胞的抑制作用。方法体外培养HUCB-MSCs,流式细胞术测表面标记;取正常人外周血,免疫磁珠分离CD3+T细胞,将分离的CD3+T与HUCB-MSCs 1∶1混合培养5 d,PHA刺激或不刺激,采用3H-TdR掺入法观察T细胞增殖,ELISA方法检测细胞因子,流式细胞术观察细胞凋亡。结果 HUCB-MSCs呈纺锤样的细胞形态,不表达CD14、CD34、CD45、HLA-DR,而表达CD29、CD44、HLA-ABC。HUCB-MSCs抑制PHA引起的T细胞增殖(5 230±550 vs 10 500±800 counts/min,P<0.001);HUCB-MSCs还能抑制异体T细胞分泌IFN-γ(510±60 vs 1 580±100 pg/mL,P<0.001)和TNF-α(590±20 vs 1 180±30 pg/mL,P<0.001),上调IL-4(16.3±8.2 vs 4.1±1.8 pg/mL,P<0.001)和IL-10(105±5 vs 17±2 pg/mL,P<0.001)分泌;HUCB-MSCs不诱导T细胞的凋亡。结论 HUCB-...
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| 关 键 词： | 脐血 间充质干细胞 T淋巴细胞 抑制 |
Mesenchymal stem cells derived from human umbilical cord blood inhibit the allogeneic peripheral blood T lymphocytes |
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| LI Zhi-bin,CAO Yin-li,LIU Si-chao,DONG Li-jie,LU Hui-ying.Mesenchymal stem cells derived from human umbilical cord blood inhibit the allogeneic peripheral blood T lymphocytes[J].Basic Medical Sciences and Clinics,2011,31(10). |
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| Authors: | LI Zhi-bin CAO Yin-li LIU Si-chao DONG Li-jie LU Hui-ying |
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| Institution: | LI Zhi-bin,CAO Yin-li,LIU Si-chao,DONG Li-jie,LU Hui-ying(Shanxi Province Woman & Child Hospital,Xi'an 710003,China) |
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| Abstract: | Objective To study the influences of mesenchymal stem cells derived from human umbilical cord blood(HUCB-MSCs) on allogeneic peripheral blood T lymphocytes.Methods HUCB-MSCs were isolated from normal human umbilical cord blood,isolated and cultured in vitro,and then surface marker was examined with FCM.After isolating CD3+T cells from normal human peripheral blood,CD3+T cells were mixed with HUCB-MSCs stimulated with PHA or not.Five days later,the effects of HUCB-MSCs on T-cell proliferation was detected by... |
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| Keywords: | umbilical cord blood mesenchymal stem cells T lymphocytes inhibition |
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