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| 桑白皮中总黄酮含量测定方法研究 | |
| 引用本文： | 段志涛,高英,霍文杰,李卫民. 桑白皮中总黄酮含量测定方法研究[J]. 中国实验方剂学杂志, 2013, 19(7): 133-135 |
| 作者姓名： | 段志涛 高英 霍文杰 李卫民 |
| 作者单位： | 广州中医药大学中药学院,广州 510006;广州中医药大学新药研究开发中心,广州 510006;广州中医药大学新药研究开发中心,广州 510006;广州中医药大学中药学院,广州 510006 |
| 摘 要： | 目的: 建立一种桑白皮中总黄酮的含量测定方法。 方法: 以桑根酮C为对照品,采用盐酸-镁粉显色体系,于481 nm下测定桑白皮总黄酮含量。 结果: 桑根酮C在146.8～734.0 mg·L-1与吸光度呈良好线性关系(n=5,r=0.999 8),平均回收率为96.74%,RSD 2.60%(n=6)。 结论: 该含量测定方法准确、简便、经济,是一种较理想的测定桑白皮中总黄酮含量的方法。 |
| 关 键 词： | 桑白皮 总黄酮 分光光度法 桑根酮C |
| 收稿时间： | 2012-06-06 |
Research on Method for Determination of Total Flavonoids from Morus alba |
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| DUAN Zhi-tao,GAO Ying,HUO Wen-jie and LI Wei-min. Research on Method for Determination of Total Flavonoids from Morus alba[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(7): 133-135 | |
| Authors: | DUAN Zhi-tao GAO Ying HUO Wen-jie LI Wei-min |
| Affiliation: | School of Chinese Material Medica, Guangzhou 510006, China;New Drug R&D Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China;New Drug R&D Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China;School of Chinese Material Medica, Guangzhou 510006, China |
| Abstract: | Objective: To establish a determination method for the extraction of total flavonoids in Morus alba. Method: The sanggenon C was used as the authentic standard. The content of total flavonids was determined by the HCl-Mg reaction colorimetry, measurement wavelength was set at 481 nm. Result: The sanggenon C calibration curve showed a good linear relationship in the ranger of 146.8-734.0 mg·L-1 (n=5, r=0.999 8) and the average recovery was 96.74%, RSD 2.60% (n=6). Conclusion: The method established was reliable and accurate. It can be used to determine the content of total flavonoid in M. alba. |
| Keywords: | Morus alba total flavonoids UV spectrophotometry sanggenon C |
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