HPLC-FLD免疫亲和柱-光衍生化法测定胖大海中黄曲霉毒素的残留量

目的：建立长柱十大功劳生物碱一测多评方法，对生物碱含量进行动态分析，确定长柱十大功劳的最佳采收期。方法：采用HPLC，以盐酸小檗碱为参照物，建立该组分与盐酸药根碱、盐酸巴马汀的相对校正因子，测定盐酸小檗碱含量，用相对校正因子计算盐酸药根碱、盐酸巴马汀含量；同时用外标法测定3种生物碱的绝对含量，比较计算值与测量值，评价一测多评的准确性；采用一测多评模式测定长柱十大功劳不同采收期的生物碱含量。结果：建立了长柱十大功劳生物碱一测多评方法，计算值与测量值无显著性差异；总碱含量4~8月逐渐升高，8月达峰值，9~12月降低。结论：该法可简便、准确测量长柱十大功劳不同生物碱含量；最佳采收期为8月。

Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization

Objective: To set up a method of multi-components quantitive by one marker to detect the content of alkaloid in Mahonia duclouxiana, research the dynamic change of alkaloid, and determine the optimal collection period. Method: By means of HPLC, determine the content of jatrorrhizine hydrochloride and pahnaline hydrochloride, using the relative correction factor (RFC) of berberine hydrochloride to them, compare with the results determined by external standard method, and use the method determine the sample at different collecting period. Result: Establish the method of multi-components quantitive by one marker, no significant difference was found between this method and the external standard method, the content of the total alkaloid increased from April to August, and was highest in August,reduced from September to December. Conclusion: The method is simple and reliable, the optima1 collection period of cultivated M. duclouxiana is August.