Inhibition and superactivation of the calcium-stimulated isoforms of adenylyl cyclase |
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Authors: | Debora Steiner Tomer Avidor-Reiss Ester Schallmach Daniella Saya and Zvi Vogel |
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Institution: | (1) Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel |
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Abstract: | It was shown previously that chronic exposure to opiate agonists increases adenylyl cyclase (AC) activity, a phenomenon termed
AC superactivation (or supersensitization). More recently, we showed that acute Gi/o-coupled receptor activation inhibits the activity of several AC isozymes, including Ca2+/calmodulin-stimulated AC-I and -VIII, whereas chronic receptor activation induces their superactivation. Here, we report
that both acute μ-opioid receptor-induced inhibition and chronic induced superactivation of AC-I and -VIII are pertussis toxin
sensitive. In addition, we show that proteins that interfere with the activity of {ie195-2} subunits ({ie195-3} scavengers)
strongly attenuate the acute inhibition of AC-I and -VIII and the superactivation of AC-I, and abolish the superactivation
of AC-VIII. Based on these results, we suggest that {ie195-4} is involved in the acute inhibition and chronic agonist-induced
superactivation of AC types I and VIII. |
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Keywords: | Opioid receptor adenylyl cyclase superactivation GTP-binding proteins addiction cAMP ionomycin {ie195-5} |
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