维生素对动脉粥样硬化危险因素损伤血管内皮的保护作用

目的:探讨4种维生素联合应用,对动脉粥样硬化危险因素损伤血管内皮是否具有保护作用。方法:24只雄性家兔被随机分成3组。对照组喂普通饲料,模型组在普通饲料中加入胆固醇、猪油和甲硫氨酸,维生素组饲料同模型组,每天再另外灌胃给予维生素E、C、B6和叶酸,连续8周。第8周末,取血检测相关指标。结果:与对照组相比,模型组血脂(除HDL-C以外)、同型半胱氨酸和内皮素水平均显著升高(t=2.412,P<0.05;t=3.802～5.830,P<0.01),血清一氧化氮虽有降低,但在两组间没有显著差异;HDL-C/LDL-C比值明显减少(t=6.622P<0.01)。与模型组比较,维生素组血清总胆固醇、LDL-C.同型半胱氨酸和血浆内皮素水平均显著降低(t=2.514～2.726,P<0.05),而血清一氧化氮显著升高,HDL-C/LDL-C比值也明显增加(t=4.128～5.076,P<0.01)。结论:维生素E、C、B6和叶酸联合运用,能够降低血中多种危险因子的水平,调节血中内皮素和一氧化氮的平衡,减少或阻断危险因素对内皮功能的损伤,对血管内皮有保护作用。

Protective effect of vitamins on vascular endothelium impaired by atherosclerosis risk factors

AIM : To investigate whether four vitamins in combination can take effects on protecting vascular endothelium from impairment in rabbits exposed to atherosclerosis risk factors.METHODS:24 white male rabbits were randomly divided into 3 groups :control group (n=6),model group (n=9) and vitamin group (n=9) . The control group was fed with normal diet ,the other two groups were fed with normal diet plus cholesterol ,lard and methionine daily. In addition, the vitamin group was given 4 vitamins by gastrogavage , the other two groups were given equal volume physiological saline for eight weeks . At the eighth week's end, the relative blood indexes were detected respectively. RESULTS: In model group, the levels of blood lipids except HDL C , homocystein and endothelin were all significantly increased , and the HDL C / LDL C rate was significantly reduced compared with control group, the serum nitric oxide level reduced but no significant difference between these two groups .In vitamin group, the levels of blood total cholesterol , LDL C, homocystein and endothelin were significantly reduced, and serum nitric oxide level was significantly increased compared with model group ,the HDL C / LDL C rate also significantly rose .