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| 隐睾下降固定术后生殖细胞的凋亡及eNOS和iNOS的表达 | |
| 引用本文： | 石金凤,莫中成,谢远杰,黄欣琼,李美香,龙治峰,贺丽萍.隐睾下降固定术后生殖细胞的凋亡及eNOS和iNOS的表达[J].中国计划生育学杂志,2010,18(2):78-81. |
| 作者姓名： | 石金凤 莫中成 谢远杰 黄欣琼 李美香 龙治峰 贺丽萍 |
| 作者单位： | 1. 南华大学显微形态实验中心,衡阳,421001 2. 南华大学组织学与胚胎学教研室,衡阳,421001 3. 湖南师范大学组织学与胚胎学教研室,衡阳,410006 |
| 摘 要： | 目的:探讨隐睾下降固定术后生精细胞的凋亡及内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)在隐睾下降固定后生精细胞中的表达。方法:将雄性SD大鼠(22日龄)随机分为隐睾组和假手术组,建立右侧隐睾模型。术后12天将隐睾组大鼠随机分为隐睾对照组和隐睾固定组,建立隐睾下降固定模型。术后第24天脱颈椎处死大鼠,取血清测定NO含量及一氧化氮合酶(NOS)活性,常规组织学切片观察各组大鼠右侧睾丸生精上皮形态,原位末端标记法(TUNEL)结合流式细胞术(FCM)检测各组睾丸生精细胞凋亡情况,Western blot和免疫组化分别检测各组大鼠右侧睾丸组织eNOS、iNOS蛋白表达的变化。结果:隐睾对照组血清中NO浓度、NOS活性以及生精细胞凋亡率最高,隐睾固定组则高于假手术组。Western blot检测发现隐睾固定组中eNOS和iNOS含量均高于假手术组,免疫组化结果显示在假手术组睾丸生精上皮中仅有eNOS和iNOS的弱表达,而隐睾固定组生精上皮中两者均有较强表达。结论:隐睾下降固定术后睾丸生精功能的改善与生精细胞凋亡减少有关。NO是隐睾下降及固定后生精细胞仍过度凋亡的重要生物活性因子。 |
| 关 键 词： | 生精细胞 凋亡 一氧化氮 隐睾下降固定术 |
Apoptosis and Expression of Endothelium Nitric Oxide Synthase and Inducible Nitric Oxide Synthase in Spermatogenic Cells of Rats Undergoing Orchidopexy |
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| Shi Jinfeng,Mo Zhongcheng,Xie Yuanjie,et al..Apoptosis and Expression of Endothelium Nitric Oxide Synthase and Inducible Nitric Oxide Synthase in Spermatogenic Cells of Rats Undergoing Orchidopexy[J].Chinese Journal of Family Planning,2010,18(2):78-81. | |
| Authors: | Shi Jinfeng Mo Zhongcheng Xie Yuanjie |
| Institution: | Shi Jinfeng,Mo Zhongcheng,Xie Yuanjie,et al.Micromorphology Laboratory Center,NanHua University,HuNan 421001 |
| Abstract: | Objective: To explore the apoptosis and expression of endothelium nitric oxide synthase(eNOS) and inducible nitric oxide synthase(iNOS) in the spermatogenic cell of the rats undergoing orchidopexy.Methods: Seventy-five immature male Sprague-Dawley rats(22 days of ages) were randomly divided into the cryptorchid and sham operation groups.The rat experimental cryptorchid model on the right side was made in the rats of the cryptorchid group.On the 12th day after operation,the cryptorchid rats were randomly div... |
| Keywords: | Spermatogenic cell Apoptosis Nitric oxide Orchidopexy |
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