重组细胞珠蛋白抗衰老与创伤愈合的初步研究

本研究利用实验室纯化得到的重组细胞珠蛋白 (rCygb), 通过人永生化角质形成细胞 (HaCAT) H₂O₂氧化应激模型、小鼠皮下连续注射D-gal导致的皮肤衰老模型、CCl₄引起的大鼠急性肝损伤模型和大鼠皮肤创伤愈合模型, 对rCygb抗氧化、增强机体抗氧化酶活性、降低氧自由基含量、延缓皮肤衰老以及创伤愈合作初步研究。结果表明: rCygb可以提高总超氧化物歧化酶 (T-SOD)、谷胱甘肽过氧化物酶 (GSH-Px) 和过氧化氢酶 (CAT) 活性; 降低乳酸脱氢酶 (LDH) 和丙氨酸氨基转移酶 (ALT) 活性; 减少丙二醛 (MDA) 含量。皮肤切片展示rCygb可以促进新生血管生成、增加胶原表达和提高抗炎能力。研究结果显示: rCygb可通过改善机体清除氧自由基的能力, 延缓皮肤衰老和促进创伤愈合。

A preliminary study of anti-aging and wound healing of recombination cytoglobin

In this paper, the preliminary study on antioxidant, enhancement of antioxidant enzymes activity, reducing the content of oxygen free radicals, delaying skin aging of the recombination cytoglobin (rCygb) purified by our lab were investigated through human keratinocyte cell line (HaCAT) H₂O₂ oxidative stress model, mouse skin aging model caused by continuous subcutaneous injection D-gal, rat acute liver injury model induced by CCl₄ and rat skin wound healing model.  The results showed that rCygb improved the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), reduced the activities of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) as well as decreased the content of malondialdehyde (MDA).  Skin biopsy showed that rCygb promoted angiogenesis, increased expression of collagen and improved the anti-inflammatory ability.  All results displayed that rCygb improved the oxygen free radical scavenging ability, delayed skin aging and promoted wound healing.