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| 天花粉提取物对HepG2.2.15细胞HBsAg和HBeAg表达的影响 | |
| 引用本文： | Chen J,Xu Z,Ouyang H,Peng M,Wu T,Liu J,Liu Y,Yan H. 天花粉提取物对HepG2.2.15细胞HBsAg和HBeAg表达的影响[J]. 中南大学学报(医学版), 2012, 37(1): 38-41. DOI: 10.3969/j.issn.1672-7347.2012.01.007 |
| 作者姓名： | Chen J Xu Z Ouyang H Peng M Wu T Liu J Liu Y Yan H |
| 作者单位： | 1. 中南大学生物科学与技术学院, 长沙 410013; 2. 湖南省第二人民医院, 长沙 410007; 3. 中南大学湘雅医院血液科, 长沙 410008 |
| 基金项目： | 国家自然科学科学基金(30971517); 中南大学“大学生创新课题”(YD09194)。 |
| 摘 要： | 目的:探讨天花粉水提取物、醇提取物对HepG2.2.15细胞的增殖及培养细胞上清中HBsAg与HBeAg表达的影响。方法:用MTT方法检测HepG2.2.15细胞的存活率,以酶联免疫法(ELISA)检测HepG2.2.15细胞上清液HBsAg和HBeAg的表达量。结果:天花粉水提取物和醇提取物处理HepG2.2.15细胞后HBsAg和HBeAg的表达均呈时间依赖性和浓度依赖性抑制,但天花粉水提物的治疗指数明显优于醇提物。结论:天花粉水提物较醇提物有更好的抗HBV活性。 |
| 关 键 词： | 天花粉 HepG2.2.15细胞 HBsAg HBeAg |
| 收稿时间： | 2011-10-24 |
Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells |
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| Chen Jia,Xu Zhaofa,Ouyang Hongtao,Peng Minyuan,Wu Ting,Liu Jing,Liu Yanping,Yan Huiwen. Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells[J]. Journal of Central South University. Medical sciences, 2012, 37(1): 38-41. DOI: 10.3969/j.issn.1672-7347.2012.01.007 | |
| Authors: | Chen Jia Xu Zhaofa Ouyang Hongtao Peng Minyuan Wu Ting Liu Jing Liu Yanping Yan Huiwen |
| Affiliation: | 1. School of Biological Science and Technology, Central South University, Changsha 410013; 2. Second People's Hospital of Hunan Province, Changsha 410007; 3. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China |
| Abstract: | Objective: To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg) in HepG2.2.15 cell supernatant. Methods: The cell survival rate of HepG2.2.15 cells was detected by MTT assay. The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay. Results: The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner. However, the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group. Conclusion: The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract. |
| Keywords: | Radix Trichosanthiszz')" href=" #" > Radix Trichosanthis HepG2.2.15 cells HBsAg HBeAg |
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