海洋来源真菌Hypocrea virens的次级代谢产物研究

目的研究海洋来源真菌Hypocrea virens发酵液的化学成分。方法采用硅胶柱色谱、Sepha-dex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等方法进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构。结果分离得到10个化合物,分别鉴定为bisdethiobis-(methylthio)glio-toxin(1)、胶霉毒素(gliotoxin,2)、5-羟基-3-羟甲基-2-甲基-7-甲氧基色原酮(5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone,3)、6-甲基苯-1,2,4-三醇(6-methyl-benzene-1,2,4-triol,4)、3β-羟基-胆甾-5-烯(3β-hydroxy-cholesta-5-ene,5)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(3-isobutyl-8-hydroxypyrrolopiperazine-2,5-dione,6)、3-苄基哌嗪-2,5-二酮(3-benzylpiperazine-2,5-dione,7)、3-苄基-8-羟基吡咯并哌嗪-2,5-二酮(3-benzyl-8-hydroxypyrrolopiper-azine-2,5-dione,8)、3S*,4R*-二羟基-3-甲基戊烷-2-酮(3S*,4R*-dihydroxy-3-methylpentan-2-one,9)、3R*,4R*-二羟基-3-甲基戊烷-2-酮(3R*,4R*-dihydroxy-3-methylpentan-2-one,10)。结论化合物1、3、4、8～10均为首次从Hypocrea属真菌中分离得到,化合物5为首次从真菌Hypocrea virens中分离得到。

Studies on the secondary metabolites from the marine-derived fungus Hypocrea virens

Objective To study chemical constituents from the fermentation broth of marine-derived fungus Hypocrea virens.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20,open ODS column chromatography and HPLC,and their structures were elucidated on the basis of spectral data and physicochemical properties.Results Ten compounds were isolated from the fermentation broth of marine-derived fungus Hypocrea virens.Their structures were identified as bisdethiobis-(methylthio)gliotoxin(1),gliotoxin(2),5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone(3),6-methylbenzene-1,2,4-triol(4),3β-hydroxy-cholesta-5-ene(5),3-isobutyl-8-hydroxypyrrolopiperazine-2,5-dione(6),3-benzyl piperazine-2,5-dione(7),3-benzyl-8-hydroxypyrrolopiperazine-2,5-dione(8),3S*,4R*-dihydroxy-3-methylpentan-2-one(9),3R*,4R*-dihydroxy-3-methylpentan-2-one(10).Conclusions Compounds 1,3,4,8-10 are isolated from the Hypocrea genus of marine-derived fungus for the first time,and compound 5 is isolated from the marine-derived fungus Hypocrea virens for the first time.