胡黄连2-C-甲基-D-赤藓糖-4-磷酸胞苷转移酶基因的生物信息学分析

目的研究胡黄连Picrorhiza kurrooa 2-C-甲基-D-赤藓糖-4-磷酸胞苷转移酶(MCT)基因特征并预测MCT结构与功能位点。方法以胡黄连MCT基因为研究对象,利用NCBI网站及生物信息学软件对其碱基分布、氨基酸组成、亲疏水性、保守区及二级结构和三级结构进行预测和分析;并对9个物种的MCT基因进行多重比对与进化分析。结果胡黄连MCT基因的mRNA序列长为1 216 bp(GenBank JQ991625.1),编码由399个氨基酸组成的蛋白质,该蛋白质相对分子质量为44 448.5,其中量最高的为Ser(10.0%);整个多肽中疏水性氨基酸占59.5%,平均疏水值为0.050;与丹参Salvia miltiorrhiza MCT氨基酸序列对比同源性最高,达99%。结论胡黄连MCT基因处于稳定状态,编码的蛋白为疏水性蛋白,在进化过程中是相对保守的,获得的保守区段序列信息为其他物种MCT基因的克隆奠定了基础。

Bio-informatics analysis of 2-C-methyl-D-erythritol 4-phosphate cytidylytransferase gene in Picrorhiza kurrooa

Objective To study the characteristics of 2-C-methyl-D-erythritol 4-phosphate cytidylytransferase (MCT) gene and to predict the structure and function site of MCT. Methods Based on MCT gene of Picrorhiza kurrooa, NCBI website and bio-informatics software were used to predict and analyze the base distribution, amino acid composition, hydrophilicity or hydrophobicity, and secondary and three-level structures of hyper-conservative region. The results were compared with MCT gene sequences in other eight species and the related evolution analysis was followed. Results The mRNA sequence of MCT gene in P. kurrooa was 1 216 bp (GenBank: JQ991625.1), coding the protein containing 399 amino acids, and the relative molecular mass of protein was 44 448.5 with 10.0% Ser in volume; In polypeptide, hydrophobic amino acid was 59.5% in volume, and the average hydropathicity was 0.050; The homology was found as high as 99% compared with MCT gene in Salvia miltiorrhiza. Conclusion The MCT gene in P. kurrooa was quite stable and the coded protein was hydrophobic, which was quite conservative in evolution; The sequence information in conservative region gained in the test provides the basis for clone ofnovel gene in other species.