贮存时间对柳叶蜡梅挥发油及桉油精含量的影响

该文采用水蒸气蒸馏法提取不同贮存时间的柳叶蜡梅挥发油,GC测定挥发油中桉油精含量,研究贮存时间对柳叶蜡梅挥发油及桉油精含量的影响,为柳叶蜡梅的质量控制提供参考。试验表明,新鲜柳叶蜡梅的挥发油含量为0.023 0 mL·g^-1,贮存4,12个月后,挥发油含量分别降为0.020 0,0.017 5 mL·g^-1;GC方法学考察的精密度、稳定性、重复性,RSD均小于2.0%,平均回收率99.50%,RSD 1.7%;新鲜提取的挥发油中桉油精含量54.30%,随着保存时间的推移,桉油精含量升高至62%左右并保持稳定。因此,贮存时间对挥发油及其中桉油精的含量有一定影响;GC测定桉油精含量准确可靠,专属性强,可重复,适合作为柳叶蜡梅的质量控制方法。

The influence of storage time on content of volatile oil and cineole of Chimonanthus salicifolius

The vapour distillation was used to extract the volatile oil of Chimonanthus salicifolius with different storage time, determine the content of cineole in volatile oil by GC, to study the influence of storage time on the content of volatile oil and cineole of C.salicifolius. We found that the content of volatile oil in fresh herbs of C.salicifolius was 0.023 0 mL·g^-1, it was decreased to 0.020 0, 0.017 5 mL·g^-1 respectively after storing for 4, 12 months; the GC methodological study of precision, stability and repeatability, RSD<2%, the average recovery rate was 99.50%, RSD 1.7%; the content of cineole in fresh volatile oil was 54.30%, it was increased to about 62% and remained stably with the time. Therefore, the content of volatile oil and cineole of C.salicifolius can change with the storage time; GC method for the determination of the content of cineole is accurate, reliable, specific and repeatable, it's suitable as a quality control method of C.salicifolius.