|
|||||
|
|
| 人胚肝低密度脂蛋白受体的提纯和鉴别 | |
| 引用本文： | 谢崇伦,蔡海江,陈秀英,陈琪. 人胚肝低密度脂蛋白受体的提纯和鉴别[J]. 南京医科大学学报(自然科学版), 1990, 0(2) |
| 作者姓名： | 谢崇伦 蔡海江 陈秀英 陈琪 |
| 作者单位： | 南京医学院病理生理学教研室，南京医学院病理生理学教研室，南京医学院病理生理学教研室，南京医学院病理生理学教研室 |
| 摘 要： | 报道了用两步法快速分离人胚肝低密度脂蛋白受体的方法。肝细胞膜用非离子型去污剂TritonX-100溶解后,经DE52纤维素离子交换层析和LDL偶联的Sepharose 4B亲和层析,将受体纯化了500倍。纯化后的低密度脂蛋白受体具有完整细胞和粗制细胞膜上受体的所有特征:(1)能与~(125)I-LDL高亲和性结合,并呈现明显的剂量效应;(2)与LDL的结合需要Ca~(2+),EDTA可阻断其结合;(3)能与抗LDL受体的单克隆抗体相互作用;(4)分子量为130kd。 |
| 关 键 词： | 低密度脂蛋白受体 胚肝 离子交换层析 亲和层析 |
PURIFICATION AND IDENTIFICATION OF LOW DENSITY LIPOPROTEIN RECEPTOR FROM HUMAN FETAL LIVERS |
|
| Xie Chonglun,et al.. PURIFICATION AND IDENTIFICATION OF LOW DENSITY LIPOPROTEIN RECEPTOR FROM HUMAN FETAL LIVERS[J]. Acta Universitatis Medicinalis Nanjing, 1990, 0(2) | |
| Authors: | Xie Chonglun et al. |
| Abstract: | We deseribe a rapid two-step procedure for the purification of the lipoprotein(LDL) receptor from human fetal livers.Liver membranes were solubilized with nonionic detergent TritonX-100.After DEAE-cellulose and LDL=Sepharose 4B affinity chromatography,the product gives a purification of about 500-fold.The purified LDL receptor retained all the properties of the receptors of intact cells and crude membranes,e.g.,(1)it bound LDL with high affinity and saturation;(2)binding of LDL to this protein was absolutely dependent on Ca~(2+) and could be (?)bolished by EDTA;(3)it interacted with the monoclonal antibody against LDL receptor;(4)having a molecular weight of 130 Kd. |
| Keywords: | low density lipoprotein receptor fetal liver DEAE-cellulose chromatography Sepharose 4B affinity chromatography |
| 本文献已被 CNKI 等数据库收录！ | |