牛白藤抗炎镇痛作用有效部位的筛选

目的比较研究牛白藤的不同溶剂提取物的抗炎镇痛作用,为牛白藤的临床应用以及筛选分离活性成分提供实验依据。方法采用二甲苯致小鼠耳廓肿胀和醋酸致小鼠毛细血管通透模型评价药物的抗炎作用,采用小鼠热刺激致痛和醋酸扭体模型评价镇痛作用,比较牛白藤水提物、醇提物及不同溶剂萃取物的抗炎镇痛作用。结果牛白藤水提物、醇提物均具有抗炎镇痛作用,同剂量之间比较以醇提物活性较强;牛白藤石油醚、乙酸乙酯萃取物能明显减轻二甲苯诱导的小鼠耳肿胀程度,抑制热刺激和醋酸引起的小鼠疼痛反应。结论牛白藤具有明显的抗炎镇痛活性,石油醚萃取物和乙酸乙酯萃取物可能是其主要有效部位。

Screening of Anti-Inflammatory and Analgesic Effective Parts of Hedvotis hedvotidea

Objective To study the anti-inflammatory and analgesic effects of different extracts of Hedyotis hedyotidea,thus to supply experimental evidence for the screening of effective parts of Hedyotis hedyotidea and their clinical use.Methods Mice models of xylene-induced ear swelling and capillary permeability test were used to evaluate anti-inflammatory activity.The analgesic activity in mice was evaluated with writhing response caused by acetic acid and with hot-plate test.The anti-inflammatory and analgesic effects were compared in water extract and alcohol extract of Hedyotis hedyotidea and the extracts extracted by different solvents.Results Both water extract and alcohol extract had anti-inflammatory and analgesic activity,but the effect of alcohol extract was stronger than that of water extract at the same dose.Petroleum ether extract and ethyl acetate extract significantly reduced ear swelling,and inhibited mice pain response induced by hot-plate stimulation and acetic acid.Conclusion Hedyotis hedyotidea has significant anti-inflammatory and analgesic activity,and petroleum ether extract and ethyl acetate extract may be the effective parts.