复方蛹虫草制剂对C57BL/6荷瘤小鼠免疫功能的影响

目的:研究蛹虫草复方制剂(CCM)对C57BL/6荷瘤小鼠免疫功能影响.方法:C57 BL/6雄性小鼠腋下皮下注入Lewis肺癌小鼠移植瘤动物模型,将荷瘤鼠分为模型组、阳性对照组以环磷酰胺ip 20 mg· kg-,复方蛹虫草制剂低、中、高剂量组(1.2,2.5,7.5)g·kg-1ig给药,连续给药14 d,以抑瘤率为指标考察CCM的体内抗肿瘤活性,分别测定其抑瘤率、T淋巴细胞亚群CD4 +/CD8+；荷瘤小鼠血清中白细胞介素-2(IL-2)、白细胞介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)水平；抗体生成细胞数以及力竭游泳时间.结果:CCM高剂量组(7.5 g·kg-1对肿瘤生长有明显的抑制作用(P＜0.05),其抑瘤率为40.3％,CCM高剂量组T淋巴细胞亚群CD3+,CD4+,CD4+/CD8+均高于模型对照组；同时中、高剂量组能显著提高荷瘤小鼠血清TNF-α,IL-2,IL-12的水平；抗体生成细胞数和力竭游泳时间也均高于荷瘤对照组(P＜0.05).结论:CCM具有提高荷瘤小鼠免疫功能的作用.

Effects of Compound Cordyceps militaris on Immune Function in C57BL/6 Tumor Bearing Mice

Objective: To study the effects of compoun Dcordyceps militaris(CCM) on immune function in C57BL/6 tumor bearing mice.Method: Transplanted Lewis tumor mice were established using subcutaneous injection of Lewis cells into C57BL/6 mice.C57BL/6 tumor bearing mice were randomly divided into five groups,namely,control group and positive group(cyclophosphamide ip 20 mg·kg-1),the low,middle and high dosage groups(ig 1.2,2.5,7.5 g·kg-1) for 14 constitutive days.The tumor inhibition rate,CD4+/CD8+ ratio,contents of interleukin(IL)-2,IL-12 and tumor necrosis factor-α(TNF-α) in tumor bearing mice serum,antibody-producing cells and forced swimming time were respectively determined.Result: CCM(7.5 g·kg-1),orally administered,had a significant inhibitory action on the growth of tumor,with the inhibition rate as 40.3%(P<0.05).CCM(7.5 g·kg-1) also improved CD3+,CD4+,CD4+/CD8+ ratio compared with the control group.Meanwhile,CCM(2.5,7.5 g·kg-1) enhanced the content of IL-2,IL-12 and TNF-α level,forced swimming time and antibody-producing cells(P<0.05).Conclusion: These findings suggested that CCM could promote the function of immunity in C57BL/6 tumor bearing mice.