银屑病脾虚证豚鼠模型构建初探

目的:探讨银屑病脾虚型病证结合动物模型的造模方法.方法:用5％普萘洛尔乳剂建立银屑病模型成功后,将24只豚鼠随机分为模型1组(银屑病组)及模型2组(银屑病脾虚组),每组12只,另设空白组12只,对模型2组豚鼠每日灌胃泻下药大黄10 mL·kg-1,2次/日,连续7d,复制脾虚模型,对各组豚鼠进行鼠耳皮损外观及病理切片观察.结果:模型2组(银屑病脾虚证组)较模型1组(银屑病组)和空白组相比,出现明显的脾虚证候,表现为活动度、进食量减少,粪便质地稀软及排便量增加等；与空白组比较,模型1组与模型2组baker评分有显著差异(P＜0.05),模型1组与模型2组比较,两组baker评分差异无统计学意义.结论:银屑病脾虚证豚鼠模型具备银屑病病理特征,并与中医脾虚证证候表现相似.

A Primary Study on Establishment of Guinea Pig Model of Psoriasis with Spleen Deficiency Syndrome

Objective: To explore and establish animal model of psoriasis combined with spleen deficiency syndrome. Method: Propranolol emulsion (5%) was used to establish psoriasis model. After the psoriasis model was successfully established, twenty-four guinea pigs were randomly divided in to two groups, psoriasis (group I, n=12) and psoriasis with spleen deficiency syndrome group (group II, n=12). Another twelve guinea pigs were used as the blank control. For establishing spleen deficiency syndrome, group II was orally given bitter and cold purgative rhubarb twice (10 mL·kg^-1) daily for a continuous duration of 7 d. Visual inspection and pathological section were carried out on ear skin lesions for all groups. Result: The total symptom scores of group II were higher than those in group I and the blank group, while the difference in Baker scores between the blank group and group I or group II appeared to be significant (P<0.05). However, the Baker scores between group I and group II showed no statistical significance. Conclusion: The psoriasis model with spleen deficiency syndrome shows the pathological features of psoriasis, and with similarities of Traditional Chinese Medicine spleen deficiency syndrome.