钠氢交换抑制剂对心肌细胞凋亡的影响

目的 探讨钠氢交换抑制剂HOE642对心肌细胞凋亡的影响。方法 以离体大鼠心脏缺血再灌注为模型,随机分成空白、对照和HOE642组,测定左室发展压、左室舒张末压、心律失常、心率和冠脉流量,TUNEL法检测细胞凋亡。结果 缺血前15min给予HOE642能明显改善心肌缺血再灌注后的心功能的恢复,减少缺血再灌注心律失常,增加复灌10分钟的冠脉流量,但对心率和复灌20、30分钟的冠脉流量无明显作用,HOE642能明显减少缺血再灌注后的细胞凋亡数量。结论HOE642对缺血再灌注损伤心肌有保护作用,能抑制缺血再灌注后的心肌细胞凋亡。

The Effect of Sodium-Hydrogen Antiporter in Preventing Myocardial Apoptosis

Objective To investigate whether the specific sodium-hydrogen antiporter HOE642 could modify ischemia and reperfusion injury and inhibit myocardium apoptosis in rat. Methods The isolated working rat hearts were divided into empty, control and HOE642 group. LVDP, LVEDP, arrythmia, coronary flow and heart rate were measured for each isolated working rat heart. After the experiment the level of apoptosis cell was evaluated by TUNEL. Results It was found that HOE642 given 15 minutes before ischemia significantly improved the recovery of the cardiac function associated with an increased coronary flow of 10 minutes after reperfusin, reduced the severity of ischemia and reperfusion arrhythmia, but had no effect on heart rate and coronary flow of 20, 30 minutes after reperfusion. At the same time, HOE642 could decrease the myocardium apoptosis during ischemia and reperrrfusion. Conclusions HOE642 has an obvious cardioprotective effects against ischemia and reperfusion. Moreover, it can inhibit myocardium apoptosis during ischemia and reperfusion.