| 半枝莲提取物抗人舌鳞癌SAS细胞增殖作用的研究 |
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| 引用本文： | 静广平,焦晓辉,王学勇,高钟秀子,杜玉滨,郑金华. 半枝莲提取物抗人舌鳞癌SAS细胞增殖作用的研究[J]. 口腔医学研究, 2009, 25(3): 249-253 |
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| 作者姓名： | 静广平 焦晓辉 王学勇 高钟秀子 杜玉滨 郑金华 |
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| 作者单位： | 哈尔滨医科大学附属口腔医院口腔颌面外科,黑龙江,哈尔滨,150001;哈尔滨医科大学解剖学教研室 |
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| 基金项目： | 国家自然科学基金资助项目(编号:305400083)教育部留学回国启动基金资助 |
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| 摘 要： | 目的：观察半枝莲提取的总黄酮（ESB）在体外对人舌鳞癌SAS细胞超微结构和增殖力的影响,探讨其可能的作用机制。方法：用0．1mg／mL,0．5mg／mL,2．5mg/mL和10．0mg／mLESB分别作用于SAS细胞24～72h,用四甲基偶氮唑盐（MTT）比色法检测细胞活性;透射电镜观察细胞超微结构的变化;免疫组化P—V两步法检测凋亡相关蛋白Bcl-2,Bax,Caspase-3用药前后表达变化。结果：ESB在体外对人舌鳞癌SAS细胞增殖有抑制作用,且呈时效及量效关系;透射电镜观察到典型凋亡细胞,线粒体等细胞器的形态变化与ESB的浓度和作用时间有关;用药后凋亡相关蛋白Bcl-2表达减弱,Bax,Caspase-3表达增强。结论：ESB可影响舌癌细胞的超微结构,降低细胞的增殖力从而抑制舌鳞癌SAS细胞过度生长;ESB诱导舌鳞癌细胞凋亡,可能与下调Bcl-2,上调Bax,Caspase-3等凋亡相关蛋白表达有关。
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| 关 键 词： | 半枝莲 总黄酮 舌癌 SAS细胞 凋亡 |
Anticancer Activity and Mechanism of Total Flavonoids Extracted from Scutellariae Barbata on Human Tongue Cancer Cell Line SAS |
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| JING Guang-ping,JIAO Xiao-hui,WANG Xue-yong,et al.. Anticancer Activity and Mechanism of Total Flavonoids Extracted from Scutellariae Barbata on Human Tongue Cancer Cell Line SAS[J]. Journal of Oral Science Research, 2009, 25(3): 249-253 |
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| Authors: | JING Guang-ping JIAO Xiao-hui WANG Xue-yong et al. |
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| Affiliation: | JING Guang-ping,JIAO Xiao-hui,WANG Xue-yong,et al.The Affiliated Stomatology Hospital of Harbin Medical University,Harbin 150001 |
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| Abstract: | Objective:To investigate the inhibition effect of total flavonoids extracted from scutellariae barbata(total flavonoids extracted from scutellariae barbata,ESB)on the proliferation of human tongue cell line SAS in vitro and explore the mechanism.Methods:SAS cell was treated by serial concentration of ESB(0.1mg/ml,0.5 mg/ml,2.5 mg/ml,10.0 mg/ml)for 24h to 72h.The inhibition activity of the ESB against SAS was estimated by MTT assay.Morphologic changes of the cell were observed under transmission electron mic... |
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| Keywords: | Scutellariae barbata Total flavonoids Tongue cancer SAS cell line Apoptosis |
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