树突细胞对肿瘤浸润淋巴细胞抗乳腺癌免疫活性影响的研究

背景与目的:树突细胞(dendriticcell,DC)又称树突状细胞,是目前已知的功能最强的抗原呈递细胞,它可以在体内、外向T淋巴细胞呈递抗原,并诱发细胞毒T淋巴细胞(cytotoxicTlymphocyte,CTL)反应。本研究旨在探讨DC激活的肿瘤浸润淋巴细胞(tumorinfiltratinglymphocytes,TIL)体外对乳腺癌细胞的杀伤活性。方法:从乳腺癌患者外周血获取DC,应用粒/巨噬细胞集落刺激因子(granulocyte/macrophagecolonystimulatingfactor,GM-CSF)、白细胞介素-4(interleukin-4,IL-4)和肿瘤抗原激活DC,然后用DC激活TIL,观察TIL在体外对自体乳腺癌细胞和Bcap-37乳腺癌细胞的杀伤活性。结果:DC激活的TIL对自体乳腺癌细胞具有很强的杀伤活性,杀伤率为(85.76±2.93)%,明显高于未经DC激活的TIL、DC激活的T淋巴细胞和未经DC激活的T淋巴细胞对自体乳腺癌细胞的杀伤率犤分别为(52.11±1.48)%、(51.35±1.46)%和(3.59±0.25)%犦。而它们对Bcap-37乳腺癌细胞的杀伤活性则相对较低犤分别为(40.03±1.29)%、(22.09±0.87)%、(21.66±0.85)%和(1.76±0.14)%犦。结论:乳腺癌患者外周血DC能诱导TIL产生高效而特异的抗乳腺癌免疫活性。

Study of anti-breast cancer activity of tumor infiltrating lymphocytes affected by dendritic cells

BACKGROUND & OBJECTIVE: Dendritic cell (DC) is the strongest antigen presenting cell(APC). It can present antigen to T lymphocytes in vivo and in vitro,and induce cytotoxic T lymphocyte(CTL) reactions.This study was designed to investigate the killing activity of tumor infiltrating lymphocytes (TILs) stimulated by dendritic cells on breast cancer cells in vitro. METHODS: DCs were isolated from peripheral blood of patients with breast cancer. DCs were stimulated by granulocyte/macrophage colony stimulating factor (GM-CSF), interleukin-4(IL-4), and tumor antigen. Then TILs were stimulated by DCs and their killing activity on autogenous breast cancer cells and Bcap-37 breast cancer cells in vitro were observed. RESULTS: TILs stimulated by DCs had very high killing activity on autogenous breast cancer cells and the killing rate was (85.76+/-2.93)%. The killing rate was higher obviously than that of TILs not stimulated by DCs and T lymphocytes stimulated by DCs or not on autogenous breast cancer cells, respectively [killing rates: (52.11+/-1.48)%, (51.35+/-1.46)%, and (3.59+/-0.25)%, respectively]. However, their killing activities on Bcap-37 breast cancer cells were lower [killing rates: (40.03+/-1.29)%, (22.09+/-0.87)%, (21.66+/-0.85)%, and (1.76+/-0.14)%, respectively]. CONCLUSION: The results indicate that DC from the patients with breast cancer can induce TIL to produce efficient and specific anti-breast cancer immune response.