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| EXPRESSION OF THE MULTIDRUG RESISTANCE GENE IN LEUKEMIC CELLS | |
| 引用本文： | 王申五,白桃,刘晓荣,单福香,江滨,赵实诚. EXPRESSION OF THE MULTIDRUG RESISTANCE GENE IN LEUKEMIC CELLS[J]. 中国癌症研究, 1993, 5(4): 285-288. DOI: 10.1007/BF03023761 |
| 作者姓名： | 王申五 白桃 刘晓荣 单福香 江滨 赵实诚 |
| 作者单位： | Institute of Hematology，Beijing Medical University，100044，Institute of Hematology，Beijing Medical University，100044，Institute of Hematology，Beijing Medical University，100044，Institute of Hematology，Beijing Medical University，100044，Institute of Hematology，Beijing Medical University，100044，Institute of Hematology，Beijing Medical University，100044 |
| 摘 要： | P-glycoprotein(Pgp; Mr=170,000) to encoded by a family of genes-Multldrug resistance gene. The Pgp has been demonstrated to mediate resistance to multiple structurally dissimilar drugs, which fuctions as an energy- dependent efflux pump so that a cell with high level of mdr expression can more effectively eliminate cytotoxic drugs. In this report, a simplified method for analysis of clinical samples and assess the level of gene expression was set up. Furthermore, by using 32P labelled mdr- 1 cDNA as the probe and RNA dot blotting the mdr-mRNAs from 5 cases of myeloblastic leukemia cells were analysed. It was shown that the level of mdr-1 expression In different myeloleukeic cells was various and reduced In one case after remission. The established method for mRNA analysis could be generalized for evaluating the level of mRNA in clinical samples. |
Expression of the multidrug resistance gene in leukemic cells |
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| Shenwu Wang,Tao Bai,Xiaorong Liu,Fuxiang Shan,Bin Jiang,Shicheng Zhao. Expression of the multidrug resistance gene in leukemic cells[J]. Chinese Journal of Cancer Research, 1993, 5(4): 285-288. DOI: 10.1007/BF03023761 | |
| Authors: | Shenwu Wang Tao Bai Xiaorong Liu Fuxiang Shan Bin Jiang Shicheng Zhao |
| Affiliation: | Wang Shenwu Bai Tao Liu XiaorongShan Fuxiang Jiang Bin Zhao ShichengInstitute of Hematology,Beijing Medical University,100044 |
| Abstract: | P-glycoprotein(Pgp; Mr=170,000) to encoded by a family of genes-Multldrug resistance gene. The Pgp has been demonstrated to mediate resistance to multiple structurally dissimilar drugs, which fuctions as an energy- dependent efflux pump so that a cell with high level of mdr expression can more effectively eliminate cytotoxic drugs. In this report, a simplified method for analysis of clinical samples and assess the level of gene expression was set up. Furthermore, by using 32P labelled mdr- 1 cDNA as the probe and RNA dot blotting the mdr-mRNAs from 5 cases of myeloblastic leukemia cells were analysed. It was shown that the level of mdr-1 expression In different myeloleukeic cells was various and reduced In one case after remission. The established method for mRNA analysis could be generalized for evaluating the level of mRNA in clinical samples. |
| Keywords: | mdr RNA Dot blottinh Myeloblastic leukemia |
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