双-三氯甲基砜对雄大鼠抗生育作用及其可逆性的研究

用略加改进的MB 50程序,进一步对双 三氯甲基砜(BTS)进行药效鉴定。结果表明,BTS10mg·kg^-1po,每周6次连服8周或30mg·kg^-1连服4周,可使SD雄鼠丧失生育力,附睾尾部精子密度,特别是精子活率,与对照比较下降非常显著(P<0.001)。给药鼠的体重、性行为、血睾酮水平以及主要内脏组织学观察均与对照无明显差异。30mg·kg^-1用药4周大鼠即不育,继续用药4周动物保持不育状态,停药4周生育力开始恢复,第6周后生育力全部恢复至正常水平。结果提示BTS有雄性抗生育作用。

STUDIES ON THE ANTIFERTILITY EFFECT OF BIS-TRICHLOROMETHYL SULFONE AND ITS REVERSIBILITY IN MALE RATS

Bis-trichloromethyl sulfone(BTS) given p.o. to male Sprague-Dawley rats at a dose of 10 mg.kg-1.d-1, 6 days a week for 8 weeks(or 30 mg.kg-1.d-1 for 4 weeks), was found to lead to infertility. The density and, in particular, the motility of the spermatozoa in the cauda epididymidis were significantly lower than those of the controls (P < 0.001). The body weight, the sex behavior, the blood testosterone level and the histology of important organs were not significantly different from those of the control rats. Fertility started to recover 4 weeks after cessation of medication and was entirely recovered 2 more weeks later in rats having received BTS for 8 weeks at the dose of 30 mg.kg-1.d-1. In vitro study showed that BTS at a concentration of 0.31 mg.ml-1 would make rat spermatozoa immobile within 20 seconds. These results indicate that BTS has both antifertility effect in male rats and direct inhibitory effect on rat spermatozoa. We conclude that nontoxic doses of BTS can induce reversible infertility in male rats.