高效液相色谱法测定蒙药复方述达格-4有效部位中山奈素和高良姜素的含量

目的：建立同时测定蒙药复方述达格-4有效部位中高良姜素、山奈素的高效液相色谱含量测定方法。方法：采用反相高效液相色谱法,色谱柱为Diamosil C18（4.6mm×250mm,5μm）,以乙腈（A）-0.5%磷酸水溶液为流动相,梯度洗脱（0～20min,34%A→37%A;20～60min,37%A）,流速1.0mL/min检测波长为286nm,柱温30℃,进样量20μL。结果：高良姜素、山奈素的线性范围分别为0.04～0.4μg（r=0.9999）,0.01656～0.1656μg（r=0.9999）;平均加样回收率（n=6）分别为99.92%、98.92%;RSD为2.09、2.33。结论：本法准确、简便,具有良好的重复性,可为蒙药复方述达格-4质量控制提供依据。

HPLC SIMULTANEOUS DETERM THE CONTENT OF KAEMPFEROL AND GALANGIN IN THE EFFECTIVE FRACTION OF SHUDAGE-4 MONGOLIAN MEDICINE COMPOUND

Objective： To develop an HPLC method for simultaneous determination of Galangin,Kaempferol in the effective fraction of shudage-4 Mongolian Medicine compound.Methods： The separation was performed on a Diamosil C18（4.6mm×250mm,5μm） column by Acetonitrile（A）-0.5%phosphoric acid（B）（0～20min,34A→37% A;20～60min,37% A） as the mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 286nm,and the column temperature was 30 ℃.Results： The calibration curves of Galangin,Kaempferol were linear in the ranges of 0.04～0.4μg（r=0.9999）,0.01656～0.1656 μg（r=0.9999） respectively;The mean recoveries（n=6） of them were 99.92% and 98.92%;RSD were 2.09,2.33.Conclusion： The developed method is simple,accurate,reliable,and is helpful to compound for Traditional Mongolian Medicine shudage-4.