| HPLC-MS/MS法测定人血浆中雷喏嗪的质量浓度 |
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| 引用本文： | 白少柏,高洪志,曲恒燕,梁宇光,李媛媛,郑专杰,王晓芳,郝光涛.HPLC-MS/MS法测定人血浆中雷喏嗪的质量浓度[J].西北药学杂志,2011,26(4):249-251. |
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| 作者姓名： | 白少柏 高洪志 曲恒燕 梁宇光 李媛媛 郑专杰 王晓芳 郝光涛 |
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| 作者单位： | 1. 解放军第五医院药剂科,宁夏,银川,750004;军事医学科学院附属医院临床药理室,北京,100071
2. 军事医学科学院附属医院临床药理室,北京,100071 |
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| 摘 要： | 目的建立HPLC-MS/MS法测定人血浆中雷喏嗪的质量浓度。方法以伏立康唑为内标,采用乙腈-体积分数0.1%甲酸水溶液(90∶10)为流动相,以Agilent-ZORBAX-C18柱(150 mm×2.1 mm,5.0μm)色谱柱为分析柱,通过电喷雾离子源(ESI),以正离子多反应监测(MRM)方式进行检测。雷喏嗪和伏立康唑用于定量分析的离子对分别为M+H]+m/z428.5→m/z279.3和M+H]+m/z350.2→m/z281.3。结果雷喏嗪线性范围为20~5 000 ng·mL-1,定量下限为20 ng·mL-1。日内和日间的RSD均小于15%,平均回收率大于75%。结论该方法专属性强,样品处理方便,灵敏度高,适用于雷喏嗪的临床药动学研究。
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| 关 键 词： | 雷喏嗪 液相色谱-质谱联用法 血浆药物质量浓度 |
Quantitative determination of ranolazine in human plasma by high performance liquid chromatography-tandem mass spectrometry |
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| BAI Shaobo,GAO Hongzhi,QU Hengyan,LIANG Yuguang,LI Yuanyuan,ZHENG Zhuanjie,WANG Xiaofang,HAO Guangtao.Quantitative determination of ranolazine in human plasma by high performance liquid chromatography-tandem mass spectrometry[J].Northwest Pharmaceutical Journal,2011,26(4):249-251. |
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| Authors: | BAI Shaobo GAO Hongzhi QU Hengyan LIANG Yuguang LI Yuanyuan ZHENG Zhuanjie WANG Xiaofang HAO Guangtao |
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| Institution: | BAI Shaobo1,2,GAO Hongzhi2,QU Hengyan2,LIANG Yuguang2,LI Yuanyuan2,ZHENG Zhuanjie2,WANG Xiaofang2,HAO Guangtao2(1.The Fifth Hospital of PLA,Ningxia Yinchuan 750004,2.Department of Clinical Pharmacology,Affiliated Hospital,Academy of Military Medical Sciences,Beijing 100071) |
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| Abstract: | Objective To establish an HPLC-MS/MS method for the determination of ranolazine in human plasma.Method Voriconazole was used as the internal standard.Ranolazine was separated on an Agilent-ZORBAX-C18 column（150 mm×2.1 mm,5.0 μm）.The mobile phase consisted of acetonitrile-0.1% formic acid（90∶10）.Electrospray ionization（ESI） was applied and operated in the positive multiple reaction monitoring（MRM） mode,by using the transitions of ＋m/z 428.5→m/z 279.3 and ＋m/z 350.2→m/z 281.3 to quantify ranolazine and the internal standard respectively.Results The linear calibration curve was obtained over the concentration range of 20-5 000 ng·mL-1.The limit of quantification was 20 ng·mL-1.The inter and intra-day precision（RSD） were less than 15%.The average recovery was above 75%.Conclusion The method is proved to be specific,convenient,sensitive and suitable for the clinical investigation of ranolazine pharmacokinetics |
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| Keywords: | ranolazine HPLC-MS/MS plasma concentration |
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