蒙药文冠木不同药用部位中槲皮素与总黄酮含量的分析比较研究

目的：对文冠木不同药用部位的槲皮素和总黄酮的含量进行比较研究。方法：测定槲皮素采用高效液相色谱法,流动相为甲醇-0.4%磷酸（61∶39）;检测波长：360nm;流速：1mL/min;柱温为室温。测定总黄酮采用紫外分光光度法,检测波长：500nm。结果：槲皮素在0.40～8.0μg/mL范围内与峰面积线性关系良好（r=0.9992）,平均加样回收率为99.00%,RSD=2.44%。总黄酮以芦丁计在8～48μg/mL范围内与吸光度呈良好的线性关系（r=0.9993）,平均加样回收率为101.3%,RSD=1.45%。槲皮素与总黄酮二者在茎枝中的含量均高于茎干。结论：该方法准确、可靠。对蒙药文冠木药材质量检控与评价提供实验依据。

ANALYSIS AND COMPARISON RESEARCH OF CONTENT OF QUERCETIN AND GENERAL FLAVONOIDES IN DIFFERENT PARTS OF XANTHOCERES SORBIFOLIA BUNGE

Objective：To compare the contents of Quercetin and General Flavonoides from different parts in Xanthoceres sorbifolia Bunge.Method： Quercetin was carried out by HPLC with the mobile phase of methanol-0.4% phosphoric acid（61∶39）.Flow rate was 1.0 mL/min.The detection wavelength was set at 360nm.Column temperature was room temperature.General Flavonoides was carried out by UV.The detection wavelength was set at 500nm.Result：The linear range of Quercetin was 0.40～8.0μg/mL（r=0.9992）,the average recovery was 99.00%,RSD was 2.44%（n=6）.The linear range of rutin was 8～48μg/mL（r=0.9993）,the average recovery was 101.3%,RSD was 1.45%（n=6）.The content of Quercetin and flavonoids branches were higher than stem in the stems.Conclusion：The method for quantitation of Quercetin and General Flavonoides of Xanthoceres sorbifolia Bunge were accurate and reliable,which can be used to apply to clinical cure.