肝细胞癌形成门静脉癌栓与DNA含量的关系

目的评估图像细胞仪（ＩＣＭ）所观察的几项参数用以揭示肝细胞癌（ＨＣＣ）形成门静脉癌栓（ＴＴＰＶ）的价值。方法９９例ＨＣＣ以ＣＡＳ┐２００型ＩＣＭ行ＤＮＡ定量分析。结果ＤＮＡ指数（ＤＩ）和增殖指数（ＰＩ）在合并ＴＴＰＶ的ＨＣＣ分别显著高于无ＴＴＰＶ的ＨＣＣ（Ｐ＜０．０５，Ｐ＜０．０１）。在同为二倍体的ＨＣＣ中，合并ＴＴＰＶ者ＰＩ值显著高于无ＴＴＰＶ者（Ｐ＜０．０５）。合并ＴＴＰＶ的ＨＣＣ，二倍体肿瘤的比例低于无ＴＴＰＶ者，而异倍体比例、细胞核面积、超５倍体细胞比例均高于无ＴＴＰＶ的ＨＣＣ，但Ｐ＞０．０５。结论随着ＤＮＡ合成水平的上升，ＨＣＣ细胞获得了侵袭门静脉、形成ＴＴＰＶ的能力；以ＩＣＭ行ＤＮＡ定量分析对揭示ＨＣＣ是否具有较强侵袭性有明显价值，其中ＰＩ是较为敏感的参数。

CORRELATION BETWEEN TUMOR THROMBOGENESIS IN PORTAL VEIN AND DNA CONTENT IN HEPATOCELLULAR CARCINOMA

Objective:Value of image cytometric parameters for predicting tumor thrombogenesis in portal vein (TTPV) in hepatocellular carcinoma (HCC)was evaluated. Methods:DNA content in 99 resected specimens of human HCC was analysed with CAS 200 image cytometry. (1)39 specimens had macroscopic TTPV,(2)30 had microscopic TTPV,and (3)30 were free of TTPV. Results:DNA index and proliferating index (PI) were significantly higher in (1)and (2) than in (3) ( P <0 05, P <0 01) In diploid HCC,PI was markedly higher in (1) and (2) than (3) ( P <0 01). Diploid rate was lower,aneuploid rate,nuclear area and super pentaploid rate were higher in (1) and (2) Conclusions:HCC cells obtained stronger invasive and metastatic capability once DNA prolifeating process increased. The DNA image cytometry has noticeable value in revealing the potency of invasiveness in HCC.