|
|||||
|
|
| 免疫磁珠法筛选人乳牙牙髓干细胞及其培养鉴定 | |
| 引用本文： | 丁祥龙,陈柯,申元源. 免疫磁珠法筛选人乳牙牙髓干细胞及其培养鉴定[J]. 南方医科大学学报, 2011, 31(5): 849-853 |
| 作者姓名： | 丁祥龙 陈柯 申元源 |
| 作者单位： | 丁祥龙,DING Xiang-long(南方医科大学南方医院口腔科,广东,广州,510515);陈柯,申元源,CHEN Ke,SHEN Yuan-yuan(广州市妇女儿童医疗中心口腔科,广东,广州,510623) |
| 摘 要： | 摘要：目的应用免疫磁珠法分离筛选人乳牙牙髓干细胞，并做培养鉴定。方法采用STRO-1为标记物以免疫磁珠分离 筛选人乳牙牙髓干细胞，观察细胞形态及生长情况，流式细胞仪检测细胞表型，并检测细胞体外多向分化能力。结果应 用免疫磁珠法筛选人乳牙牙髓细胞可获得乳牙牙髓干细胞，经统计学处理证明看其生长速度慢于牙髓细胞，细胞表型分 析证实CD29、CD105高表达，CD34、CD45低表达。矿化诱导和成脂诱导证实STRO-1+乳牙牙髓细胞具有干细胞多向分 化的能力。结论免疫磁珠法是有效的分离纯化人乳牙牙髓干细胞的方法。所分离的细胞具有干细胞的表型特点及多向 分化能力。 |
| 关 键 词： | 乳牙 牙髓 干细胞 分化 免疫磁珠 |
Isolation and identification of stem cells derived from human exfoliated deciduous teeth by magnetic activated cell sorting |
|
| DING Xiang-long,CHEN Ke,SHEN Yuan-yuan. Isolation and identification of stem cells derived from human exfoliated deciduous teeth by magnetic activated cell sorting[J]. Journal of Southern Medical University, 2011, 31(5): 849-853 | |
| Authors: | DING Xiang-long CHEN Ke SHEN Yuan-yuan |
| Affiliation: | DING Xiang-long1,CHEN Ke2,SHEN Yuan-yuan2 1Department of Stomatdogy Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,2Department of Stomatology,Guangzhou Women and Children's Medical Center,Guangzhou 510623 |
| Abstract: | Objective To isolate stem cells from human exfoliated deciduous teeth(SHEDs) and identify their phenotypes and multi-lineage differentiation potential. Methods Human pulp tissue from exfoliated deciduous teeth were dissected and digested to obtain the single cell suspension. The SHEDs selected by magnetic activated cell sorting system(MACS) were identified by examination of the cell morphology and growth in vitro and detection of the expressions of the cell markers. Osteogenic and adipogenic induction was p... |
| Keywords: | deciduous teeth dental pulp stem cells differentiation immunomagnetic beads |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 | |
| 点击此处可从《南方医科大学学报》下载全文 | |