非小细胞肺癌Survivin基因表达与细胞增殖的相关性研究

目的 探讨非小细胞肺癌Survivin基因表达与细胞增殖的相互关系。方法 应用免疫组织化学方法检测了76例非小细胞肺癌组织、12例癌旁组织和12例肺良性病变组织中Survivin基因和核增殖相关抗原Ki-67的表达水平，并分析两者的相互关系。结果 非小细胞肺癌患者Survivin基因和Ki-67的蛋白表达水平分别为80、26％(61／76)和69、74％(53／76)，极明显高于对照组(P＜0．01)。两者间的表达呈明显的正相关(r=0．42，P＜0．01)。结论 不同性质的肺病变组织Survivin基因的表达水平不同，可以用作非小细胞肺癌临床诊断的可靠指标和基因治疗的目的基因。Survivin基因的过度表达与非小细胞肺癌的细胞增殖活性明显相关，Survivin基因可能具有促进肺癌细胞增殖的活性。

Correlation between expression of Survivin gene and cell proliferation in non-small cell lung cancer

Objective To investigate the correlation between expression of Survivin gene and cell proliferation in non-small cell lung cancer.Methods Expression of Survivin gene and Ki-67 was detected by immunohistochemical techniques in 76 cases of non-small cell lung cancer (squamous carcinoma: 48 cases; adenocarcinoma: 28 cases), the adjacent tumor tissues and 12 cases of benign lesions, respectively.Results In non-small cell lung cancer, the expression rate of Survivin gene and Ki-67 index in protein level was 80.26 % and 69.74 %, respectively, significantly higher than in the control group (P< 0.01). There was a significantly positive correlation between the expression of Survivin gene in protein level and that of Ki-67 index in non-small cell lung cancer tissues (r= 0.42, P< 0.01). Conclusion There was overexpression of Survivin gene in non-small cell lung cancer tissues. As a specific biomarker of malignant tumors, Survivin gene may also serve as a definite diagnostic marker for non-small cell lung cancer, and may also as an important target in cancer gene therapy. The over-expression of Survivin gene may promote proliferative activities.