减毒结核分枝杆菌H37 Ra感染小鼠模型的实验研究

目的：：研究减毒结核分枝杆菌(M. tb)H37Ra 感染小鼠模型及其病理损伤的机制。方法：将清洁级Balb/c小鼠分为对照组和M. tb模型组，各3只。将H37Ra按照每只小鼠1×106 CFU的剂量，经尾静脉注射感染小鼠，分别于感染7、14和21 d后，无菌操作取小鼠肺和脾脏组织，测量肺和脾脏的脏器系数，匀浆后行活菌培养；肺和脾脏组织冷冻切片，行HE染色观察病理损伤情况。结果：小鼠感染M. tb 14和21 d后，脾脏脏器系数均较感染7 d后显著升高(P<0.01和P<0.05)。感染7 d后，肺和脾脏组织匀浆均可检出活菌，脾脏组织H37Ra活菌量明显高于肺脏，并随感染时间逐渐增加；感染14 d后，肺组织病理观察可见明显感染性病变损伤。结论：M. tb H37Ra 经尾静脉注射感染小鼠模型可造成部分结核病样病理性损伤。

Study on the mouse model infected by Mycobacterium tuberculosis H37 Ra

Objective:To Study on the pathological lesion in Balb/c mice infected with H37Ra. Methods:The mice were divided into control group and model group infected with Mycobacterium tuberculosis. Balb/c mice was injected via the tail vein with attenuated Mycobacterium tuberculosis H37Ra (1 × 106 CFU/mouse). After infection,living bacteria,organ weight index of lung or spleen,and histopathological lesion were detected after 7,14,and 21 days of infection. Results:Compared with the group of control,the organ coefficient of spleen increased statistically after 14 days or 21 days of infection(P<0. 01 and P<0. 05);The number of living bacteria in spleen tissue of infected mice increased with the days of infection and was higher in spleen tissue than that in lung tissue. Tuberculosis-like lesions were observed in spleen and lung tissue of infected mice model. Conclusions:Attenuated Mycobacterium tuberculosis H37Ra could induced tuberculosis-like lesions in Balb/c mouse via the tail vein injection.