精液分析中精子密度室内质量控制方法研究

目的建立一种可行的精液密度测定的质量控制方法.方法采用血细胞计数板和计算机辅助精液分析(CASA)两种方法,对所选取的与精子头部直径相近的质控珠进行高、中、低三种密度测定,分别于日内及日间测试12次.结果采用血细胞计数板方法测试高、中、低三种密度结果的日内变异系数分别为12.09%、8.86%、4.72%,日间变异系数分别为16.71%、10.65%、10.22%.CASA法日内变异系数分别为6.65%、5.93%、3.80%,日间变异系数分别为7.90%、6.84%、6.14%.结论质控珠可用于以上两种测试精子密度方法的室内质量控制,从而达到保证实验室测试精子密度质量的目的.

The study of internal quality control on the sperm density in semen analysis

Objective To set up a feasible technique of internal quality control on the sperm density. Methods:Using the two methods of blood-cell counter and computer assistant sperm analysis(CASA),12 times separately in every day and 12 days we detect the density of quality control bead of low,moderate,high density and the diameter of quality control bead is similar with that of a sperm. Results:Using the blood-cell counter,the intraday CVs of low,moderate,high density were 12.09%,8.86%,4.72% and extraday CVs of low,moderate,high density were 16.71%,10.65%,10.22%. Using CASA , the intraday CVs of low,moderate,high density were 6.65%,5.93%,3.80%,and extraday CVs of low,moderate,high density were 7.90%,6.84%,6.14%. Conclusions:The quality control bead can be used in internal quality control on the sperm density for the two methods.That can help to assure the quality of the analysis on sperm density.