|
|||||
|
|
| 高效液相色谱法测定石淋通颗粒中夏佛塔苷的含量 | |
| 引用本文： | 冉亚东,越亮. 高效液相色谱法测定石淋通颗粒中夏佛塔苷的含量[J]. 海峡药学, 2014, 0(9): 66-67 |
| 作者姓名： | 冉亚东 越亮 |
| 作者单位： | 太极集团重庆涪陵制药厂有限公司重庆408000 |
| 摘 要： | 目的建立了高效液相色谱法测定石淋通颗粒中夏佛塔苷含量的测定方法。方法采用DiamonsilTM C18色谱柱（5μm,4.6×250μg）,甲醇-0.10%磷酸溶液（32∶68）为流动相,流速：1.0mL·min-1,检测波长272nm,柱温：35℃。结果夏佛塔苷进样量在0.006μg~1.044μg范围内与峰面积呈良好线性（r=0.9999）,平均回收率为99.53%,RSD=1.05%（n=6）。结论该方法操作简便、准确、重复性好,可用于石淋通颗粒的质量控制。 |
| 关 键 词： | 石淋通颗粒 夏佛塔苷 高效液相色谱法 |
Determination of Schaftoside in Shilintong Granules by HPLC |
|
| RAN Ya-dong,YUE liang. Determination of Schaftoside in Shilintong Granules by HPLC[J]. Strait Pharmaceutical Journal, 2014, 0(9): 66-67 | |
| Authors: | RAN Ya-dong YUE liang |
| Affiliation: | ( Taiji Group Chongqing fuling Pharmaceutical Co., Ltd. Chongqing 408000, China) |
| Abstract: | OBJECTIVE To establish a HPLC method to determine the content of schaftoside in Shilintong Granules.METHODS The DiamonsilTM C18 （5μm,4.6 ×250mm） column was used.The mobile phase consisted of methanol-0.10%phosphoric acid （25∶75） at 1.0mL· min -1.The detection wavelength was 272nm and the column temperature was 35℃.RESULTS The calibration curve was in good linearity within 0.006 ~1.044μg （ r =0.9999 ）.The average recovery was 99.53%,RSD was 1.05%（ n=6 ）.CONCLUSION The method is simple ,ac-curate and reproducible.It can be used for content determination of schaftoside in Shilintong Granules. |
| Keywords: | Shilintong Granules Schaftoside HPLC |
| 本文献已被 维普 等数据库收录！ | |