单发胃癌和食管/胃双原发癌中胃癌CDH1基因甲基化研究

目的研究单发胃癌和食管/胃双原发癌中胃癌组织CDH1基因启动子区CpG岛甲基化变化及其临床意义。方法应用甲基化特异性PCR法,检测62例单发胃癌、30例食管/胃双原发癌中胃癌组织、癌旁组织及18例正常胃组织CDH1基因甲基化情况。结果单发胃癌和食管/胃双原发癌中胃癌组织CDH1基因甲基化率分别为61.3%和76.7%,癌旁组织分别为22.6%和23.3%,正常胃组织为0。单发胃癌和食管/胃双原发癌患者癌组织CDH1基因甲基化率与癌旁组织、正常组织比较,差异均有统计学意义(P<0.05),癌旁组织CDH1基因甲基化率与正常组织比较,差异均无统计学意义(P>0.05)。单发胃癌与食管/胃双原发癌中胃癌组织CDH1基因甲基化阳性率比较,差异无统计学意义(P>0.05)。单发胃癌和食管/胃双原发癌患者CDH1基因甲基化与性别、年龄、烟酒史和上消化道肿瘤家族史均无关(P>0.05)。结论 CDH1基因甲基化在单发胃癌和食管/胃双原发癌胃癌中是一常见的表观遗传学事件,可能是一个独立的危险因素。

Comparative Study of CDH1 Gene Methylation in Single and Double Primary Cancer of Gastric Adenocarcinoma

Objective To study the changes of CDH1 gene promoter CpG island methylation and its clinical significance in patients with single gastric cancer(SGC) and gastric cancer(GC)in esophagus/stomach double primary cancers(ESDC).Methods 62 patients with SGC,30 patients with GC of ESDC and 18 healthy persons were assigned to be checked CDH1 gene Methylation of cancerous tissues,adjacent non-cancerous tissues and normal health gastric tissues by methylation specific polymerase chain reaction(MSP).Results The rates of CDH1 gene Methylation in the cancerous tissues were 61.3% and 76.7% respectively,and 22.6% and 23.3% respectively in adjacent non-cancerous tissues in patients with SGC and GC of ESDC,and 0 in normal gastric tissues.There were significant differences between the cancerous tissues and adjacent non-cancerous tissues,normal gastric tissues(P all<0.05).And the differences between adjacent non-cancerous tissues and normal tissues was not significant(P all >0.05).The difference of positive rate of CDH1 gene Methylation between cancerous tissues in SGC and those in GC of ESDC was not statistically significant(P>0.05).There were no relationship between CDH1 gene methylation and sex,age,tobacco-alcohol history and family history of upper gastrointestinal cancer(ΜGIC)(P all >0.05).Conclusion CDH1 gene methylation in SGC and GC in ESDC is a common epigenetic genetics event.It may be an independent risk factor.