Growth inhibition of human glioma cells modulated by retrovirus gene transfection with antisense IL-8 |
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| Authors: | Ryuya Yamanaka Ryuichi Tanaka Seiichi Yoshida Takafumi Saitoh Kazuyuki Fujita |
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| Institution: | (1) Department of Neurosurgery, Brain Research Institute, Niigata, Japan;(2) Department of Obstetrics and Gynecology, Niigata University, Niigata, Japan;(3) LCMB, NCI, NIH, Building 37, Room 1E24, 20852 Bethesda, MD, USA |
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| Abstract: | The human glioma cell line, NP-1, expresses IL-8 mRNA and constitutively secretes IL-8 protein. Administration of recombinant IL-8 increased the proliferation of NP-1 cells in a dose-dependent manner. In the presence of anti-IL-8 antiserum, the IL-8 mediated proliferation of NP-1 cell growth was inhibited. Further, NP-1 cell growth was inhibited by transfection of retroviral constructs encoding antisense IL-8 bothin vitro andin vivo models. These results suggest that antisense IL-8 gene therapy could be beneficial in gliomas where autocrine stimulation by IL-8 is implicated. |
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| Keywords: | glioma IL-8 antisense IL-8 retrovirus gene transfer |
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