| 森林脑炎病毒RNA的提取及其性质 |
| |
| 引用本文： | 杨佩英,司炳银,黄志尚. 森林脑炎病毒RNA的提取及其性质[J]. 山东大学学报(医学版), 1987, 0(4) |
| |
| 作者姓名： | 杨佩英 司炳银 黄志尚 |
| |
| 作者单位： | 军事医学科学院微生物流行病学研究所(杨佩英，司炳银)，军事医学科学院微生物流行病学研究所(黄志尚) |
| |
| 摘 要： | 从森林脑炎病毒感染的鼠脑组织中直接抽提总核酸,然后经Sepharose4B柱层析将病毒RNA与细胞RNA分离,方法简便、分离的病毒RNA在260nm处有典型的紫外吸收光谱,260/280nm的比值近似2,对RNase敏感,有感染性。
|
| 关 键 词： | 脑炎病毒 RNA 鼠脑 |
STUDIES ON TICK-BORNE ENCEPHALITIS VIRUS (TBE) GENOME RNA ISOLATION AND PROPERTY |
| |
| Yang Peiying Inst. of Microbiol. and Epidemiol. Military Acad. of Med. Sci. STUDIES ON TICK-BORNE ENCEPHALITIS VIRUS (TBE) GENOME RNA ISOLATION AND PROPERTY[J]. Journal of Shandong University:Health Sciences, 1987, 0(4) |
| |
| Authors: | Yang Peiying Inst. of Microbiol. and Epidemiol. Military Acad. of Med. Sci |
| |
| Affiliation: | Yang Peiying Inst. of Microbiol. and Epidemiol. Military Acad. of Med. Sci |
| |
| Abstract: | Total RNA was extracted from the infectious brain. ViralRNA was chromatogra phed by sepharose 4B column Infectious virus RNAwas eluted from the column This method is useful for large-scale isolationof TBE virus RNA. The viral RNA obtained can be used in the oligonucleo-tide fingerprinting analysis and genetic engineering. |
| |
| Keywords: | Encephalitis viruses RNA: Murine brain |
| 本文献已被 CNKI 等数据库收录！ |
