| 基于毒素多肽BmK NaTx-13的全蝎质控工艺研究 |
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| 引用本文： | 陈永根,桑明,卢悟广,王明远,刘金满,陈胜君,李松,胡春萍,王奇,曹鹏. 基于毒素多肽BmK NaTx-13的全蝎质控工艺研究[J]. 南京中医药大学学报, 2020, 36(4): 509-516 |
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| 作者姓名： | 陈永根 桑明 卢悟广 王明远 刘金满 陈胜君 李松 胡春萍 王奇 曹鹏 |
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| 作者单位： | 1.广州中医药大学科技创新中心，广东 广州 510405 |
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| 摘 要： | 目的 基于毒素多肽建立一种新型的全蝎质量检测工艺。方法 从东亚钳蝎毒液中分离得到了一种新型钠通道毒素BmK NaTx-13，制备并鉴定了BmK NaTx-13的特异性抗体，并通过竞争性ELISA法检测全蝎提取物中的BmK NaTx-13的含量。结果 建立的竞争性酶联免疫吸附法可快速检测全蝎提取物中的BmK NaTx-13的含量并呈现良好的浓度线性依赖。结论 该研究为全蝎质控提供了一种快速免疫检测方法，为提升全蝎入药质量标准提供了借鉴。
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| 关 键 词： | 全蝎 BmK NaTx-13 ELISA 质控工艺 |
Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13 |
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| CHEN Yong-gen,SANG Ming,LU Wu-guang,WANG Ming-yuan,LIU Jin-man,CHEN Sheng-jun,LI Song,HU Chun-ping,WANG Qi,CAO Peng. Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13[J]. Journal of Nanjing University of Traditional Chinese Medicine(Natural Science), 2020, 36(4): 509-516 |
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| Authors: | CHEN Yong-gen SANG Ming LU Wu-guang WANG Ming-yuan LIU Jin-man CHEN Sheng-jun LI Song HU Chun-ping WANG Qi CAO Peng |
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| Abstract: | OBJECTIVE To develop a BmK Scorpion quality control technology based on toxin peptide. METHODS In this study， a novel sodium channel toxin BmK NaTx-13 was isolated from BV， and the specific polyclonal antibody against BmK NaTx-13 was obtained by immunizing rabbits. RESULTS A competitive enzyme linked immunosorbent assay (ELISA) based on BmK NaTx-13 specific antibody could detect the content of BmK NaTx-13 in the processed BmK scorpion extract and showed a good linear concentration dependent. CONCLUSION This research provided a rapid immunoassay method for establishing the quality detection processed BmK scorpion， and provided an insight for improving the quality standard of whole scorpion medicine. |
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| Keywords: | BmK scorpion BmK NaTx-13 ELISA quality control |
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