| 体外连续培养人口腔粘膜角化细胞 |
| |
| 引用本文： | 周海文,周曾同,商庆新,曹谊林. 体外连续培养人口腔粘膜角化细胞[J]. 中华口腔医学杂志, 2000, 35(6): 455-457,I034 |
| |
| 作者姓名： | 周海文 周曾同 商庆新 曹谊林 |
| |
| 作者单位： | 1. 200011,上海第二医科大学口腔医学院口腔内科
2. 上海第二医科大学组织工程研究中心上海市组织工程重点实验室 |
| |
| 摘 要： | 目的 建立一个稳定的人正常口腔粘膜角化细胞体外培养体系。方法 取正常口腔粘膜，经Ｄｉｓｐａｓｅ及胰蛋白酶消化获取细胞，采用无血清培养液进行原代及传代培养，并进行形态学观察和角蛋白免疫组化染色等一系列细胞定性研究。结果 获取的细胞为单一上皮细胞；细胞可连续传４～５代，成活３０～５０ｄ；细胞呈铺路石状，为上皮细胞的典型形态；电镜下见细胞具有丰富的张力丝和桥粒等特征性超微结构；角蛋白免疫组化染色阳性。结
|
| 关 键 词： | 细胞培养 口腔粘膜 角蛋白 角化细胞 癌前病变 |
Serial cultivation of normal human oral keratinocytes |
| |
| H Zhou,Z Zhou,Q Shang. Serial cultivation of normal human oral keratinocytes[J]. Chinese journal of stomatology, 2000, 35(6): 455-457,I034 |
| |
| Authors: | H Zhou Z Zhou Q Shang |
| |
| Affiliation: | Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghan 200011, China. |
| |
| Abstract: | OBJECTIVE: To establish a method of culturing normal human oral keratinocytes. METHODS: Specimens obtained from healthy humans undergoing oral surgery were dissociated by Dispase and trypsin into a single cell suspension. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope (EM). Cytokeratins were showed by immunohistochemistry. RESULTS: The cells could be maintained in culture up to 4-5 passages, or 30-50 days. EM revealed that there were desmosomes and tonofibrils in the culture cells. The cells showed positive staining for cytokeratin antibody. CONCLUSIONS: The culture technique for growing normal human oral keratinocytes has been established. |
| |
| Keywords: | Cell culture Mouth mucosa Keratin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
