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| 流式细胞术用于药物抗真菌效应的检测 | |
| 引用本文： | 李庆,陈多炎,翟志敏. 流式细胞术用于药物抗真菌效应的检测[J]. 临床输血与检验, 2007, 9(3): 213-216 |
| 作者姓名： | 李庆 陈多炎 翟志敏 |
| 作者单位： | 230001,合肥,安徽医科大学附属省立医院中心实验室;230001,合肥,安徽医科大学附属省立医院检验科 |
| 基金项目： | 安徽省立医院院助课题项目（No.2005YZ06）资助 |
| 摘 要： | 目的尝试建立用流式细胞术检测药物抗真菌效应的新方法。方法以碘化丙锭为荧光染料,用流式细胞术检测标准菌株近平滑念珠菌ATCC22019在不同浓度氟康唑（FCZ）作用下各管平均荧光强度,探讨最佳实验条件。结果真菌MFI值随着FCZ浓度的增加而增强,其变化具有浓度依赖性,较好地反映了药物的抗真菌效应。结论成功建立用流式细胞术检测药物抗真菌效应的新方法,流式细胞术在该领域的应用值得进一步研究。 |
| 关 键 词： | 流式细胞术 近平滑念珠菌 氟康唑 抗真菌效应 |
| 文章编号： | 1671-2587（2007）03-0213-04 |
| 修稿时间： | 2006-11-21 |
Flow Cytometry: a Method of Testing the Sensitivty of Fungi to Fluconaiole |
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| LI Qing,CHEN Duo-yan,Zhai Zhi-min. Flow Cytometry: a Method of Testing the Sensitivty of Fungi to Fluconaiole[J]. Journal of Clinical Transfusion and Laboratory Medicine, 2007, 9(3): 213-216 | |
| Authors: | LI Qing CHEN Duo-yan Zhai Zhi-min |
| Affiliation: | Central Laboratory, Department of Clinical Laboratory, Anhui Provincial Hospital, Affiliated to Anhui Medical Univosity, Hefei 230001 |
| Abstract: | Objective To develop a new method for drug antifungal effect by flow cytomtry (FCM). Methods ATCC 22019 of Candida parapsilosis were fluorescence labeled by propidium iodide (PI), mean fluorescence intensity (MFI) under different concentrations of fluconazole(FCZ) were detected by flow cytometry,and explore the best experiment condition. Results With drug concentrains rising,the MFI of fungi were increasing,the change trend was concentration-dependent, which reflected the drug antifungal effect exactly. Conclusion It was successful to develop a new method for drug antifungal effect by flow cytometry,the application of FCM in this field is worth studying deeply. |
| Keywords: | Flow cytometry Candida parapsilosis Fluconazole Antifungal effect |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |